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NMR metabolomics reveals effects of Cryptosporidium infections on host cell metabolome

Miller, Christopher N., Panagos, Charalampos G., Mosedale, William, Kvac, Martin, Howard, Mark J., Tsaousis, Anastasios D (2019) NMR metabolomics reveals effects of Cryptosporidium infections on host cell metabolome. Gut Pathogens, 11 (13). ISSN 1757-4749. (doi:10.1186/s13099-019-0293-x) (KAR id:73548)


Background: Cryptosporidium is an important gut microbe whose contributions towards infant and immunocompromise

patient mortality rates are steadily increasing. Over the last decade, we have seen the development of

various tools and methods for studying Cryptosporidium infection and its interactions with their hosts. One area that is

sorely overlooked is the effect infection has on host metabolic processes.

Results: Using a 1H nuclear magnetic resonance approach to metabolomics, we have explored the nature of the

mouse gut metabolome as well as providing the first insight into the metabolome of an infected cell line. Statistical

analysis and predictive modelling demonstrated new understandings of the effects of a Cryptosporidium infection,

while verifying the presence of known metabolic changes. Of note is the potential contribution of host derived

taurine to the diarrhoeal aspects of the disease previously attributed to a solely parasite-based alteration of the gut

environment, in addition to other metabolites involved with host cell catabolism.

Conclusion: This approach will spearhead our understanding of the Cryptosporidium-host metabolic exchange and

provide novel targets for tackling this deadly parasite.

Item Type: Article
DOI/Identification number: 10.1186/s13099-019-0293-x
Uncontrolled keywords: Cryptosporidiosis, NMR, Metabolomics, COLO-680N, Taurine
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Anastasios Tsaousis
Date Deposited: 19 Apr 2019 09:28 UTC
Last Modified: 28 Jul 2022 22:09 UTC
Resource URI: (The current URI for this page, for reference purposes)

University of Kent Author Information

Miller, Christopher N..

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Mosedale, William.

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Howard, Mark J..

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Tsaousis, Anastasios D.

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