Browning, Douglas F., Godfrey, Rita E., Richards, Kirsty L., Robinson, Colin, Busby, Stephen J.W. (2019) Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production. Biochemical Society Transactions, 47 (2). pp. 755-763. ISSN 0300-5127. (doi:10.1042/BST20190059) (KAR id:73490)
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| Official URL: https://doi.org/10.1042/BST20190059 |
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Abstract
The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give controlled expression that is suitable for diverse biotechnology applications.
| Item Type: | Article |
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| DOI/Identification number: | 10.1042/BST20190059 |
| Uncontrolled keywords: | Escherichia coli, bacterial transcription initiation, lac promoter, tac promoter, Lac repressor, recombinant protein production |
| Subjects: | Q Science > QP Physiology (Living systems) > QP517 Biochemistry |
| Divisions: | Divisions > Division of Natural Sciences > Biosciences |
| Depositing User: | Susan Davies |
| Date Deposited: | 15 Apr 2019 15:08 UTC |
| Last Modified: | 05 Nov 2024 12:36 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/73490 (The current URI for this page, for reference purposes) |
University of Kent Author Information
Robinson, Colin.
| Creator's ORCID: |
 https://orcid.org/0000-0002-1739-032X
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| CReDIT Contributor Roles: |
- Link to SensusAccess
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