Skip to main content

Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production

Browning, Douglas F., Godfrey, Rita E., Richards, Kirsty L., Robinson, Colin, Busby, Stephen J.W. (2019) Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production. Biochemical Society Transactions, 47 (2). pp. 755-763. ISSN 0300-5127. (doi:10.1042/BST20190059) (KAR id:73490)

PDF Author's Accepted Manuscript
Language: English
Download (583kB) Preview
[thumbnail of Kudinov et al 2019 BMC Genomics 20 270.pdf]
Preview
This file may not be suitable for users of assistive technology.
Request an accessible format
Official URL
https://doi.org/10.1042/BST20190059

Abstract

The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give controlled expression that is suitable for diverse biotechnology applications.

Item Type: Article
DOI/Identification number: 10.1042/BST20190059
Uncontrolled keywords: Escherichia coli, bacterial transcription initiation, lac promoter, tac promoter, Lac repressor, recombinant protein production
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Susan Davies
Date Deposited: 15 Apr 2019 15:08 UTC
Last Modified: 10 Apr 2020 23:00 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/73490 (The current URI for this page, for reference purposes)
Robinson, Colin: https://orcid.org/0000-0002-1739-032X
  • Depositors only (login required):

Downloads

Downloads per month over past year