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Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production

Browning, Douglas F., Godfrey, Rita E., Richards, Kirsty L., Robinson, Colin, Busby, Stephen J.W. (2019) Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production. Biochemical Society Transactions, 47 (2). pp. 755-763. ISSN 0300-5127. (doi:10.1042/BST20190059) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

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Abstract

The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give controlled expression that is suitable for diverse biotechnology applications.

Item Type: Article
DOI/Identification number: 10.1042/BST20190059
Uncontrolled keywords: Escherichia coli, bacterial transcription initiation, lac promoter, tac promoter, Lac repressor, recombinant protein production
Subjects: Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Sue Davies
Date Deposited: 15 Apr 2019 15:08 UTC
Last Modified: 19 Jun 2019 13:49 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/73490 (The current URI for this page, for reference purposes)
Robinson, Colin: https://orcid.org/0000-0002-1739-032X
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