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Application of Imaging Flow Cytometry for the Characterization of Intracellular Attributes in Chinese Hamster Ovary Cell Lines at the Single Cell Level

Pekle, Eva, Smith, Andrew, Rosignoli, Guglielmo, Sellick, Christopher, Smales, Christopher Mark, Pearce, Claire (2019) Application of Imaging Flow Cytometry for the Characterization of Intracellular Attributes in Chinese Hamster Ovary Cell Lines at the Single Cell Level. Biotechnology Journal, . p. 1800675. ISSN 1860-6768. (doi:10.1002/biot.201800675) (KAR id:73370)

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Abstract

Biopharmaceutical manufacturing using Chinese hamster ovary (CHO) cells requires the

fluorescence activated cell sorting (FACS) has the potential to combine isolation of single cells

identifying cell lines with desirable phenotypes for manufacturing. This study describes the

cell level to identify cell attributes predictive of productivity. IFC assays to quantify organelle

single cells were developed. The assays were then validated against orthogonal standard flow

used in cell line development and show how cellular properties can be correlated with

process. Our analysis found HC polypeptide and mRNA to be predictive of productivity early

productivity.

Item Type: Article
DOI/Identification number: 10.1002/biot.201800675
Uncontrolled keywords: Chinese hamster ovary (CHO) cells, Single cell imaging, Imaging flow cytometry, organelles
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Mark Smales
Date Deposited: 04 Apr 2019 10:16 UTC
Last Modified: 29 Mar 2020 00:00 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/73370 (The current URI for this page, for reference purposes)
Smales, Christopher Mark: https://orcid.org/0000-0002-2762-4724
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