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Investigation of Sterol uptake by a new Beta-Cyclodextrin Porous Network

Ahmad, Towseef Ishmum (2018) Investigation of Sterol uptake by a new Beta-Cyclodextrin Porous Network. Master of Science by Research (MScRes) thesis, University of Kent,. (KAR id:72963)

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Abstract

This report details the synthesis and analysis of new β-cyclodextrin (β-CD) networks: KβCD-MOF-1, KβCD-MOF-1-Chol and KβCD-MOF-2. Investigations of uptake of four sterols have also been conducted. The syntheses of the crystals were achieved by a vapour diffusion method at ambient temperature and pressure using mixtures of β-CD/potassium hydroxide in a ratio of 1:20, β-CD/potassium hydroxide/cholesterol in a ratio of 1:20:⅓ and β-CD/potassium benzoate in a ratio of 1:1, respectively. The analysis of crystals was performed using nuclear magnetic resonance (NMR) spectroscopy, X-ray diffraction (XRD) and optical and scanning electron microscopy. XRD showed KβCD-MOF-1 was made of accessible β-CD channels with each layer being perpendicular to the one above and the one below it. KβCD-MOF-1-Chol had a honeycomb like structure with cholesterol present in the β-CD channels. KβCD-MOF-2 was similar to a previously reported inclusion complex, although the crystallisation methods used were different.

Four types of sterols (Cholesterol, testosterone, beta-estradiol and deoxycholic acid) were used to investigate the uptake ratio of KβCD-MOF-1 and this was achieved using proton NMR analysis. The results showed that uptake of these sterols were successful. The NMR results also showed an inverse relation between numbers of polar groups in the sterol to the uptake by KβCD-MOF-1 network. The ratios of sterols:β-CD were observed to be 1:2, 1:4, 1:2 and 1:3 for cholesterol, testosterone, beta-estradiol and deoxycholic acid, respectively.

The encapsulations of UiO-66 crystals in to vesicles were investigated using fluorescence and electron microscopy. Although fluorescence microscopy provided some evidence of encapsulation however it was not confirmed by electron microscopy positively. Therefore the results remain inconclusive.

Item Type: Thesis (Master of Science by Research (MScRes))
Thesis advisor: Blight, Barry
Thesis advisor: Rossman, Jeremy
Uncontrolled keywords: Chemistry, Biology, Metal Organic Frameworks, MOFs, Porous Crystals, Vesicles, Porous Networks, Beta-Cyclodextrin, Potassium, CDMOF, Proton NMR, Vapour diffusion crystallisation, KβCD-MOF-1, KβCD-MOF-1-Chol, KβCD-MOF-2, Sterol, Cholesterol, Beta-estradiol, Testosterone, Deoxycholic acid, Microscopy
Divisions: Faculties > Sciences > School of Physical Sciences
SWORD Depositor: System Moodle
Depositing User: System Moodle
Date Deposited: 13 Mar 2019 12:10 UTC
Last Modified: 03 Jun 2019 09:32 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/72963 (The current URI for this page, for reference purposes)
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