Skip to main content

Direct Single-Cell Analysis of Human Polar Bodies and Cleavage-Stage Embryos Reveals No Evidence of the Telomere Theory of Reproductive Ageing in Relation to Aneuploidy Generation

Turner, Kara Jane, Lynch, Colleen, Rouse, Hannah, Vasu, Vimal, Griffin, Darren K. (2019) Direct Single-Cell Analysis of Human Polar Bodies and Cleavage-Stage Embryos Reveals No Evidence of the Telomere Theory of Reproductive Ageing in Relation to Aneuploidy Generation. Cells, 8 (2). p. 163. ISSN 2073-4409. (doi:10.3390/cells8020163) (KAR id:72860)

PDF Publisher pdf
Language: English


Creative Commons Licence
This work is licensed under a Creative Commons Attribution 4.0 International License.
Download (626kB) Preview
[img]
Preview
Official URL
https://doi.org/10.3390/cells8020163

Abstract

Reproductive ageing in women, particularly after the age of 35, is associated with an exponential increase in the proportion of chromosomally abnormal oocytes produced. Several hypotheses have attempted to explain this observation, including the ‘limited oocyte pool’ hypothesis and the ‘two-hit’ hypothesis, the latter explaining that a depletion in oocyte quality with age results from the multiple opportune stages for errors to occur in meiosis. Recently however, the telomere theory of reproductive ageing in women has been proposed. This suggests that shortened telomeres in oocytes of women of advanced maternal age render oocytes unable to support fertilization and embryogenesis. Despite a credible rationale for the telomere theory of reproductive ageing in women, very few studies have assessed telomere length directly in human oocytes or preimplantation embryos. Therefore, we directly assessed relative telomere length in first polar bodies and blastomeres from cleavage stage (day 3) embryos. In both cell types we tested the hypothesis that (1) older women have shorter telomeres and (2) chromosomally abnormal (aneuploid) gametes/embryos have shorter telomeres. In all cases, we found no evidence of altered telomere length associated with age-related aneuploidy.

Item Type: Article
DOI/Identification number: 10.3390/cells8020163
Uncontrolled keywords: telomere length; reproductive ageing; first polar bodies; blastomeres; aneuploidy
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Darren Griffin
Date Deposited: 06 Mar 2019 11:12 UTC
Last Modified: 03 Jun 2019 09:31 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/72860 (The current URI for this page, for reference purposes)
Griffin, Darren K.: https://orcid.org/0000-0001-7595-3226
  • Depositors only (login required):

Downloads

Downloads per month over past year