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Generation of microalga Chlamydomonas reinhardtii expressing shrimp antiviral dsRNA without supplementation of antibiotics

Charoonnart, Patai, Worakajit, Nichakorn, Zedler, Julie Annemarie Zita, Meetam, Metha, Robinson, Colin, Saksmerprome, Vanvimon (2019) Generation of microalga Chlamydomonas reinhardtii expressing shrimp antiviral dsRNA without supplementation of antibiotics. Scientific Reports, 9 (1). Article Number 3164. ISSN 2045-2322. (doi:10.1038/s41598-019-39539-x) (KAR id:72824)

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Abstract

RNA interference (RNAi) is an efective way of combating shrimp viruses by using sequence-specifc

microalgae expressing antiviral dsRNA as a sustainable feed supplement for shrimp ofering viral

Chlamydomonas reinhardtii for the expression of a dsRNA cassette targeting a shrimp yellow head

the generation of stable, marker-free C. reinhardtii transformants without the supplementation of

trial to evaluate the efciency of the algal RNAi-based vaccine against the virus. Shrimps treated

(dpi), whereas 84.1% mortality was observed in control groups exposed to the YHV virus. RT-PCR

(55.6±11.1%) compared to control groups (88.9±11.1% and 100.0±0.0%, respectively). Our

protective feedstock in shrimp aquaculture.

Item Type: Article
DOI/Identification number: 10.1038/s41598-019-39539-x
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Colin Robinson
Date Deposited: 01 Mar 2019 16:20 UTC
Last Modified: 22 Sep 2020 16:08 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/72824 (The current URI for this page, for reference purposes)
Robinson, Colin: https://orcid.org/0000-0002-1739-032X
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