Skip to main content

Bacterial sensors define intracellular free energies for correct enzyme metalation

Osman, Deenah, Martini, Maria Alessandra, Foster, Andrew W., Chen, Junjun, Scott, Andrew J. P., Morton, Richard J., Steed, Jonathan W., Lurie-Luke, Elena, Huggins, Thomas G., Lawrence, Andrew D., and others. (2019) Bacterial sensors define intracellular free energies for correct enzyme metalation. Nature Chemical Biology, 15 . pp. 241-249. ISSN 1552-4450. E-ISSN 1552-4469. (doi:10.1038/s41589-018-0211-4) (KAR id:72280)

PDF Author's Accepted Manuscript
Language: English
Download (672kB) Preview
[img]
Preview
Official URL
https://doi.org/10.1038/s41589-018-0211-4

Abstract

There is a challenge for metalloenzymes to acquire their correct metals because some inorganic elements form more stable complexes with proteins than do others. These preferences can be overcome provided some metals are more available than others. However, while the total amount of cellular metal can be readily measured, the available levels of each metal have been more difficult to define. Metal-sensing transcriptional regulators are tuned to the intracellular availabilities of their cognate ions. Here we have determined the standard free energy for metal complex formation to which each sensor, in a set of bacterial metal sensors, is attuned: The less competitive the metal, the less favourable the free energy and hence greater availability to which the cognate allosteric mechanism is tuned. Comparing these free energies with values derived from the metal affinities of a metalloprotein reveals the mechanism of correct metalation exemplified here by a cobalt-chelatase for vitamin B12.

Item Type: Article
DOI/Identification number: 10.1038/s41589-018-0211-4
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Martin Warren
Date Deposited: 11 Feb 2019 10:49 UTC
Last Modified: 06 Feb 2020 04:19 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/72280 (The current URI for this page, for reference purposes)
Lawrence, Andrew D.: https://orcid.org/0000-0002-5853-5409
Warren, Martin J.: https://orcid.org/0000-0002-6028-6456
  • Depositors only (login required):

Downloads

Downloads per month over past year