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The Effect of Fluorescent tagging of 1,2-Propanediol Utilization Microcompartment Shell Proteins on the Shell Formation and Spatial Organization Within the Bacterial Cell

Packwood, Sarah (2017) The Effect of Fluorescent tagging of 1,2-Propanediol Utilization Microcompartment Shell Proteins on the Shell Formation and Spatial Organization Within the Bacterial Cell. Doctor of Philosophy (PhD) thesis, University of Kent,. (KAR id:72060)

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Abstract

Bacterial microcompartments are isohedral structures composed entirely of protein and contain a

carry out a specific metabolic reaction. There are seven essential shell proteins encoded on the

having a critical role in the formation of the compartment. Other genes in the Pdu operon interact

which has been shown to form filament-like structures. These filaments only appear in the presence

the spatial distribution of microcompartments. This study has explored the interaction between

be PduK, and to characterise the mechanism of this interaction PduK truncations were generated

microcompartments to form was examined, as well as the ineraction between PduV and these

directed mutagenesis of the GTP binding site, which was then examined by microscopic and

individual shell protein was tagged with mCherry. Microscopy techniques such as live cell imaging

were able to form correctly, if PduV filament formation was affected in any way and whether this

work presented has given insight into the formation of the microcompartment shell, higlighting the

effect this can have on the formation of the shell. It has also revealed possible previously

the modification of shell proteins affecting PduV filaments. Finally, it has given greater evidence to

previous suggestions that PduV may be a GTPase.

Item Type: Thesis (Doctor of Philosophy (PhD))
Thesis advisor: Warren, Martin
Divisions: Faculties > Sciences > School of Biosciences
SWORD Depositor: System Moodle
Depositing User: System Moodle
Date Deposited: 29 Jan 2019 11:11 UTC
Last Modified: 23 Jan 2020 04:15 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/72060 (The current URI for this page, for reference purposes)
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