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Nucleic acid amplification mediated microbial identification

Bruce, Ian J. (1993) Nucleic acid amplification mediated microbial identification. Science Progress, 77 (3-4). pp. 183-206. ISSN 0036-8504. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
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Abstract

Nucleic acid amplification techniques have and are revolutionising the way research, academia, medicine, environmental and quality control issues are dealt with. In particular, the polymerase chain reaction, (PCR), originally mentioned in 1985 has greatly modified the ways in which scientists and clinicians go about nucleic acid sequence analysis and manipulation and has great implications for molecular biology in general and diagnostics in particular. This report attempts to review the use of PCR and isothermal nucleic acid amplification techniques in the rapid identification of microorganisms. Key methodologies have been noted, their strengths and weaknesses commented upon and comparisons have been drawn between isothermal and thermally cycled nucleic amplification techniques in rapid microbial identification.

Item Type: Article
Uncontrolled keywords: bacterial DNA; bacterial RNA; DNA modification methylase EcoRI; ribosome DNA; RNA 16S; site specific DNA methyltransferase (adenine specific), bacterial infection; bacterium; computer program; Escherichia coli; genetics; human; methodology; microbiology; molecular genetics; nucleotide sequence; polymerase chain reaction; review, Bacteria; Bacterial Infections; Base Sequence; DNA, Bacterial; DNA, Ribosomal; Escherichia coli; Human; Molecular Sequence Data; Polymerase Chain Reaction; RNA, Bacterial; RNA, Ribosomal, 16S; Site-Specific DNA-Methyltransferase (Adenine-Specific); Software; Support, Non-U.S. Gov't
Subjects: Q Science > QR Microbiology
Divisions: Faculties > Sciences > School of Physical Sciences
Faculties > Sciences > School of Physical Sciences > Functional Materials Group
Depositing User: I. Bruce
Date Deposited: 30 Jan 2019 16:49 UTC
Last Modified: 30 May 2019 08:50 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/71988 (The current URI for this page, for reference purposes)
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