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Electrophoretic karyotype of the amylolytic yeast Lipomyces starkeyi and cloning, sequencing and chromosomal localization of its TRP1 gene

Bignell, G.R., Bruce, Ian J., Evans, I.H. (1996) Electrophoretic karyotype of the amylolytic yeast Lipomyces starkeyi and cloning, sequencing and chromosomal localization of its TRP1 gene. Current Genetics, 30 (1). pp. 83-88. ISSN 0172-8083. (doi:10.1007/s002940050104) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:71984)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1007/s002940050104

Abstract

The genome of the amylolytic yeast strain Lipomyces starkeyi NCYC 1436 was analysed using contour-clamped homogeneous electric field gel electrophoresis (CHEF). The banding pattern under a variety of running conditions indicating the presence of 11 different chromosome-sized DNA molecules. The sizes of these chromosome bands were determined by comparison with chromosomes from standard strains of Schizosaccharomyces pombe and Saccharomyces cerevisiae. The chromosomal bands were estimated to be within the range 0.7-2.8 Mb, with the genome (excluding mitochondrial DNA) estimated at 15 Mb. The molecular cloning of the TRP1 gene, isolated from a genomic library of this strain, is also reported: the gene was present on a 6.5-kb Sau3A DNA fragment, and complemented the trpC gene of E. coli. The DNA sequence was determined (EMBL accession No. Z68292) and compared to other tryptophan biosynthetic genes encoding N-(5'-phosphoribosyl) anthranilate isomerase (PRAI) activity. The gene was also used as a probe in hybridization studies, and by this means, its chromosomal location was identified.

Item Type: Article
DOI/Identification number: 10.1007/s002940050104
Uncontrolled keywords: dna fragment; fungal dna; isomerase; tryptophan, amino acid synthesis; article; chromosomal localization; chromosome banding pattern; comparative study; dna probe; dna sequence; escherichia coli; fungal genetics; gel electrophoresis; gene isolation; gene library; genetic complementation; karyotype; molecular cloning; nonhuman; priority journal; saccharomyces cerevisiae; schizosaccharomyces pombe; yeast, Aldose-Ketose Isomerases; Amino Acid Sequence; Base Sequence; Chromosome Mapping; Cloning, Molecular; DNA, Fungal; Electrophoresis, Gel, Pulsed-Field; Fungal Proteins; Genes, Fungal; Karyotyping; Molecular Sequence Data; Saccharomyces cerevisiae Proteins; Saccharomycetales; TATA Box, Escherichia coli; Lipomyces starkeyi; Saccharomyces cerevisiae; Schizosaccharomyces pombe
Subjects: Q Science
Q Science > QR Microbiology
Divisions: Faculties > Sciences > School of Physical Sciences
Faculties > Sciences > School of Physical Sciences > Functional Materials Group
Depositing User: I. Bruce
Date Deposited: 30 Jan 2019 16:07 UTC
Last Modified: 30 May 2019 08:50 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/71984 (The current URI for this page, for reference purposes)
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