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A novel plasmid pIJB1 possessing a putative 2,4-dichlorophenoxyacetate degradative transposon Tn5530 in Burkholderia cepacia strain 2a

Xia, X.-S., Aathithan, S., Oswiecimska, K., Smith, A.R.W., Bruce, Ian J. (1998) A novel plasmid pIJB1 possessing a putative 2,4-dichlorophenoxyacetate degradative transposon Tn5530 in Burkholderia cepacia strain 2a. Plasmid, 39 (2). pp. 154-159. ISSN 0147-619X. (doi:10.1006/plas.1997.1332) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:71979)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1006/plas.1997.1332

Abstract

A 102-kb plasmid, pIJB1, was isolated from Burkholderia cepacia strain 2a, which is able to use 2,4-dichlorophenoxyacetate (2,4-D) as a sole carbon source, and a physical map of the plasmid has been established. It was observed that spontaneous loss of a 37-kb fragment of the plasmid after growth in nonselective medium occurred, generating a plasmid of diminished size, pIJB2. The deletion event is concomitant with the loss of the 2,4-D dissimilatory phenotype, indicating that at least some of the 2,4-D degradative genes are on the missing fragment. The missing fragment is flanked by two identical 4.3-kb insertion sequences (IS) and shows a typical composite transposon structure of 41-kb in size, designated Tn5530. The mutant plasmid pIJB2 possesses a single copy of the IS element.

Item Type: Article
DOI/Identification number: 10.1006/plas.1997.1332
Uncontrolled keywords: 2,4 dichlorophenoxyacetic acid; antibiotic agent; DNA fragment; xenobiotic agent, agar gel electrophoresis; article; Burkholderia cepacia; gene; nonhuman; phenotype; plasmid; polymerase chain reaction; restriction mapping; transposon, Burkholderia; Burkholderia cepacia; Burkholderia cepacia; Negibacteria
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > School of Physical Sciences
Depositing User: Ian Bruce
Date Deposited: 31 Jan 2019 07:23 UTC
Last Modified: 16 Feb 2021 14:02 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/71979 (The current URI for this page, for reference purposes)
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