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Measles virus RNA is not detected in inflammatory bowel disease using hybrid capture and reverse transcription followed by the polymerase chain reaction

Chadwick, N., Bruce, Ian J., Schepelmann, S., Pounder, R.E., Wakefield, A.J. (1998) Measles virus RNA is not detected in inflammatory bowel disease using hybrid capture and reverse transcription followed by the polymerase chain reaction. Journal of Medical Virology, 55 (4). pp. 305-311. ISSN 0146-6615. (doi:10.1002/(SICI)1096-9071(199808)55:4<305::AID-JMV9>3.0.CO;2-4) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1002/(SICI)1096-9071(199808)5...

Abstract

Recent epidemiological and immunohistochemical studies have indicated a possible link between measles virus and inflammatory bowel disease (IBD). The aim of this study was to use a sensitive and robust method for the detection of measles virus RNA in IBD and control clinical samples. Peripheral blood mononuclear cells and intestinal resection tissue from IBD and control patients were studied. Two methods were used to determine the presence of measles virus RNA: hybrid capture, using measles virus-specific oligonucleotides linked to paramagnetic solidphase supports, was carried out on total cellular RNA to enrich for measles virus RNA sequences. Reverse transcription followed by the polymerase chain reaction (RT-PCR) using rTth DNA polymerase was employed for amplification of measles virus N-gene sequences amongst the enriched species. Total RNA was also used for RT-PCR of a housekeeping mRNA species to assess RNA quality. RT-PCR for another region of the measles genome (the haemagglutinin (H) gene) was also undertaken in order to confirm the results obtained using N-gene primers for analysis of these samples. None of the samples were positive for measles N- or H-gene RNA using RT-PCR. Positive control samples confirmed the sensitivity of the methods employed. These results show that either measles virus RNA was not present in the samples, or was present below the sensitivity limits known to have been achieved.

Item Type: Article
DOI/Identification number: 10.1002/(SICI)1096-9071(199808)55:4<305::AID-JMV9>3.0.CO;2-4
Uncontrolled keywords: virus rna, article; blood analysis; clinical article; clinical trial; controlled study; crohn disease; enteritis; human; human tissue; measles virus; reverse transcription polymerase chain reaction; rna analysis; technique; ulcerative colitis, Adolescent; Adult; Aged; Blotting, Southern; Child; Colitis; Colitis, Ulcerative; Crohn Disease; Female; Humans; Inflammatory Bowel Diseases; Intestines; Leukocytes, Mononuclear; Male; Measles virus; Middle Aged; Polymerase Chain Reaction; RNA, Viral; Transcription, Genetic, Measles virus; RNA viruses
Subjects: R Medicine > RB Pathology
Divisions: Faculties > Sciences > School of Physical Sciences
Faculties > Sciences > School of Physical Sciences > Functional Materials Group
Depositing User: I. Bruce
Date Deposited: 31 Jan 2019 07:18 UTC
Last Modified: 30 May 2019 08:49 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/71978 (The current URI for this page, for reference purposes)
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