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PCR detection of genetically modified soya and maize in foodstuffs

Hurst, C.D., Knight, A., Bruce, Ian J. (1999) PCR detection of genetically modified soya and maize in foodstuffs. Molecular Breeding, 5 (6). pp. 579-586. ISSN 1380-3743. (doi:10.1023/A:1009654623025) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1023/A:1009654623025

Abstract

The detection of genetically modified foodstuffs is becoming both a food sales and legal necessity. This study reports a rapid DNA extraction/PCR-based method for the detection of genetically modified soya (GMS) and maize (GMM) in mixed samples of transgenic and unmodified soybeans and maize kernels, and a variety of processed samples including soya flour, soya protein isolates, extruded defatted soya, acid- and alcohol-precipitated soya concentrates, soya lecithin, maize grits, seasoned corn puffs and salted corn chips. The presence of GMS DNA was determined with two pairs of primers directed towards different GMS target sequences and GMM by one primer pain. In addition, a multiplex PCR reaction which utilises an internal positive control was developed for both genetically modified organisms (GMOs). Results indicated that the methods are sensitive and specific enough to detect GMS down to a level of 0.01 dry weight in single-product PCRs and 0.1 in multiplex PCRs and GMM down to 0.001 dry weight in single-product PCRs and 0.01 in multiplex PCR. The methods are considered to represent a viable route for the commercial detection of GMS and GMM in foodstuffs.

Item Type: Article
DOI/Identification number: 10.1023/A:1009654623025
Uncontrolled keywords: detection; dry weight; food product; genetically modified food; maize; polymerase chain reaction; protein product; soybean; transgenic plant
Divisions: Faculties > Sciences > School of Physical Sciences
Faculties > Sciences > School of Physical Sciences > Functional Materials Group
Depositing User: I. Bruce
Date Deposited: 30 Jan 2019 17:38 UTC
Last Modified: 30 May 2019 08:49 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/71972 (The current URI for this page, for reference purposes)
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