Application of magnetite and silica-magnetite composites to the isolation of genomic DNA

Taylor, J.I., Hurst, C.D., Davies, M.J., Sachsinger, N., Bruce, Ian J. (2000) Application of magnetite and silica-magnetite composites to the isolation of genomic DNA. Journal of Chromatography A, 890 (1). pp. 159-166. ISSN 0021-9673. (doi:10.1016/S0021-9673(00)00107-2) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Abstract

Magnetite and silica-magnetite composites were used as adsorbents for the isolation of genomic DNA from maize kernels. Two methods are described for the preparation of silica-magnetite composites, both of which afford higher yields of genomic DNA than when using magnetite alone, or a commerically available kit. DNA isolated using silica-magnetite was suitable for use in further applications such as polymerase chain reaction amplification and enzyme digestion.

Item Type:	Article
DOI/Identification number:	10.1016/S0021-9673(00)00107-2
Uncontrolled keywords:	magnetite; silicon dioxide; sorbent, analytic method; article; DNA determination; maize; nonhuman; polymerase chain reaction; priority journal, DNA Restriction Enzymes; DNA, Plant; Electrophoresis, Agar Gel; Ferrosoferric Oxide; Iron; Oxides; Polymerase Chain Reaction; Silicon Dioxide; Zea mays, Zea mays
Subjects:	Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology
Divisions:	Faculties > Sciences > School of Physical Sciences Faculties > Sciences > School of Physical Sciences > Functional Materials Group
Depositing User:	I. Bruce
Date Deposited:	01 Feb 2019 13:02 UTC
Last Modified:	30 May 2019 08:49 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/71970 (The current URI for this page, for reference purposes)