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Simultaneous direct detection of Salmonella spp., Listeria Monocytogenes and Escherichia Coli o157 in milk samples by magnetic extraction and multiplex PCR

Omiccioli, E., Amagliani, G., Brandi, G., Bruce, Ian J., Magnani, M. (2009) Simultaneous direct detection of Salmonella spp., Listeria Monocytogenes and Escherichia Coli o157 in milk samples by magnetic extraction and multiplex PCR. Journal of Rapid Methods and Automation in Microbiology, 17 (2). pp. 195-213. ISSN 1060-3999. (doi:10.1111/j.1745-4581.2009.00170.x) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:71943)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1111/j.1745-4581.2009.00170.x

Abstract

The aim of our work was to develop a new molecular method for the simultaneous detection of Salmonella spp., Listeria monocytogenes and Escherichia coli O157 directly in milk samples. Three specific target sequences were chosen for a multiplex polymerase chain reaction (PCR) assay: a 155-bp region of the Salmonella spp. tetrathionate reductase (ttr) locus; a 173-bp region of the L. monocytogenes listeriolysin O gene (hlyA); a 217-bp region of the E. coli O157 lipopolysaccharide gene (rfbE). An internal amplification control was also included to detect PCR inhibition. In addition, a magnetic-based extraction method was also developed to isolate PCR-ready DNA from milk. The assay was able to detect, whether alone or mixed, also with a difference of 2 log units, as few as 102 cells of each pathogen per 10 mL of spiked milk.

Item Type: Article
DOI/Identification number: 10.1111/j.1745-4581.2009.00170.x
Uncontrolled keywords: Escherichia coli; Listeria monocytogenes; Salmonella
Subjects: Q Science > QR Microbiology
Divisions: Faculties > Sciences > School of Physical Sciences
Faculties > Sciences > School of Physical Sciences > Functional Materials Group
Depositing User: Ian Bruce
Date Deposited: 31 Jan 2019 08:25 UTC
Last Modified: 30 May 2019 08:49 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/71943 (The current URI for this page, for reference purposes)
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