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Mg2+ -free ATP regulates the processivity of native cytoplasmic dynein

Behrens, Vincent A., Walter, Wilhelm J., Peters, Carsten, Wang, Tianbang, Brenner, Bernhard, Geeves, Michael A., Scholz, Tim, Steffen, Walter (2019) Mg2+ -free ATP regulates the processivity of native cytoplasmic dynein. FEBS Letters, 593 (3). pp. 296-307. ISSN 0014-5793. (doi:10.1002/1873-3468.13319) (KAR id:71765)

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Cytoplasmic dynein, a microtubule?based motor protein, is responsible for many cellular functions ranging from cargo transport to cell division. The various functions are carried out by a single isoform of cytoplasmic dynein, thus requiring different forms of motor regulation. A possible pathway to regulate motor function was revealed in optical trap experiments. Switching motor function from single steps to processive runs could be achieved by changing Mg2+ and ATP concentrations. Here, we confirm by single molecule total internal reflection fluorescence microscopy that a native cytoplasmic dynein dimer is able to switch to processive runs of more than 680 consecutive steps or 5.5 ?m. We also identified the ratio of Mg2+?free ATP to Mg.ATP as the regulating factor and propose a model for dynein processive stepping.

Item Type: Article
DOI/Identification number: 10.1002/1873-3468.13319
Uncontrolled keywords: cytoplasmic dynein, Mg2+?free ATP, processivity, regulation, single molecule experiment
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Michael Geeves
Date Deposited: 22 Jan 2019 13:02 UTC
Last Modified: 21 Dec 2019 00:00 UTC
Resource URI: (The current URI for this page, for reference purposes)
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