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Chromosome map of the Siamese cobra: did partial synteny of sex chromosomes in the amniote represent “a hypothetical ancestral super-sex chromosome” or random distribution?

Singchat, Worapong, O’Connor, Rebecca, Tawichasri, Panupong, Suntronpong, Aorarat, Sillapaprayoon, Siwapech, Suntrarachun, Sunutcha, Muangmai, Narongrit, Baicharoen, Sudarath, Peyachoknagul, Surin, Chanhome, Lawan, and others. (2018) Chromosome map of the Siamese cobra: did partial synteny of sex chromosomes in the amniote represent “a hypothetical ancestral super-sex chromosome” or random distribution? BMC Genomics, 19 . Article Number 939. ISSN 1471-2164. (doi:10.1186/s12864-018-5293-6) (KAR id:71533)

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https://doi.org/10.1186/s12864-018-5293-6

Abstract

Background

We produced a detailed chromosome map of the Siamese cobra genome, focusing on chromosome 2 and sex chromosomes. Synteny of the Siamese cobra chromosome 2 (NKA2) and NKAZ were highly conserved among snakes and other squamate reptiles, except for intrachromosomal rearrangements occurring in NKA2. Interestingly, twelve BACs that had partial homology with sex chromosomes of several amniotes were mapped on the heterochromatic NKAW as hybridization signals such as repeat sequences. Sequence analysis showed that most of these BACs contained high proportions of transposable elements. In addition, hybridization signals of telomeric repeat (TTAGGG)n and six microsatellite repeat motifs ((AAGG)8, (AGAT)8, (AAAC)8, (ACAG)8, (AATC)8, and (AAAAT)6) were observed on NKAW, and most of these were also found on other amniote sex chromosomes.

Conclusions

The frequent amplification of repeats might involve heterochromatinization and promote sex chromosome differentiation in the Siamese cobra W sex chromosome. Repeat sequences are also shared among amniote sex chromosomes, which supports the hypothesis of an ancestral super-sex chromosome with overlaps of partial syntenies. Alternatively, amplification of microsatellite repeat motifs could have occurred independently in each lineage, representing convergent sex chromosomal differentiation among amniote sex chromosomes.

Item Type: Article
DOI/Identification number: 10.1186/s12864-018-5293-6
Uncontrolled keywords: amniote, synteny, repeat sequences, sex chromosome, snake
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Darren Griffin
Date Deposited: 04 Jan 2019 10:19 UTC
Last Modified: 06 Oct 2020 19:34 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/71533 (The current URI for this page, for reference purposes)
O’Connor, Rebecca: https://orcid.org/0000-0002-4270-970X
Griffin, Darren K.: https://orcid.org/0000-0001-7595-3226
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