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The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase.

Schubert, Heidi L., Raux-Deery, Evelyne, Brindley, Amanda A., Leech, Helen K., Wilson, Keith S., Hill, Christopher P., Warren, Martin J. (2002) The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase. EMBO Journal, 21 (9). pp. 2068-2075. ISSN 0261-4189. E-ISSN 1460-2075. (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:71)

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Abstract

Sirohaem is a tetrapyrrole-derived prosthetic group that is required for the essential assimilation of sulfur and nitrogen into all living systems as part of the sulfite and nitrite reductase systems. The final two steps in the biosynthesis of sirohaem involve a beta-NAD(+)-dependent dehydrogenation of precorrin-2 to generate sirohydrochlorin followed by ferrochelation to yield sirohaem. In Saccharomyces cerevisiae, Met8p is a bifunctional enzyme that carries out both of these reactions. Here, we report the 2.2 A resolution crystal structure of Met8p, which adopts a novel fold that bears no resemblance to the previously determined structures of cobalt- or ferro-chelatases. Analysis of mutant proteins suggests that both catalytic activities share a single active site, and that Asp141 plays an essential role in both dehydrogenase and chelatase processes.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences > Protein Science Group
Depositing User: Susan Davies
Date Deposited: 19 Dec 2007 17:53 UTC
Last Modified: 25 Jun 2020 03:00 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/71 (The current URI for this page, for reference purposes)
Raux-Deery, Evelyne: https://orcid.org/0000-0002-8189-678X
Warren, Martin J.: https://orcid.org/0000-0002-6028-6456
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