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Direct binding of Talin to Rap1 is required for Cell-ECM adhesion in Drosophila

Camp, D., Haage, A., Solianova, V., Castle, W. M., Xu, Q.A., Lostchuck, Emily, Goult, Benjamin T, Tanentzapf, Guy (2018) Direct binding of Talin to Rap1 is required for Cell-ECM adhesion in Drosophila. Journal of cell science, 131 (24). ISSN 0021-0953. E-ISSN 1477-9137. (doi:10.1242/jcs.225144) (KAR id:70281)

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Attachment of cells to the Extracellular Matrix (ECM) via integrins is essential for animal development and tissue maintenance. The cytoplasmic protein Talin is necessary for linking integrins to the cytoskeleton and its recruitment is a key step in the assembly of the adhesion complex. However, the mechanisms that regulate Talin recruitment to sites of adhesion in vivo are still not well understood. Here we show that Talin recruitment to, and maintenance at, sites of integrin-mediated adhesion requires a direct interaction between Talin and the GTPase Rap1. A mutation that blocks the direct binding of Talin to Rap1 abolished Talin recruitment to sites of adhesion and the resulting phenotype phenocopies null alleles of Talin. Moreover, we show that Rap1 activity modulates Talin recruitment to sites of adhesion via its direct binding to Talin. These results identify the direct Talin-Rap1 interaction as a key in vivo mechanism for controlling integrin-mediated cell-ECM adhesion.

Item Type: Article
DOI/Identification number: 10.1242/jcs.225144
Uncontrolled keywords: Talin, Rap1, Drosophila, Integrin
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Ben Goult
Date Deposited: 22 Nov 2018 12:08 UTC
Last Modified: 09 Dec 2022 06:05 UTC
Resource URI: (The current URI for this page, for reference purposes)
Goult, Benjamin T:
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