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Far-reaching cellular consequences of tat deletion in Escherichia coli revealed by comprehensive proteome analyses

Dolata, Katarzyna M., Montero, Isabel Guerrero, Miller, Wayne, Sievers, Susanne, Sura, Thomas, Wolff, Christian, Schlüter, Rabea, Riedel, Katharina, Robinson, Colin (2019) Far-reaching cellular consequences of tat deletion in Escherichia coli revealed by comprehensive proteome analyses. Microbiological Research, 218 . pp. 97-107. ISSN 0944-5013. (doi:10.1016/j.micres.2018.10.008) (KAR id:70187)

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Abstract

In Escherichia coli, the Twin-arginine translocation (Tat) pathway secretes a set of folded proteins

secretion impairs outer membrane integrity and leads to decreased cell growth. Only recently, the

applications in the production of fully folded heterologous proteins. In this study, we investigated

comprehensive proteomic analysis revealed activation of several stress responses and

observed that a tat deletion triggers protein aggregation, membrane vesiculation, synthesis of

disturbs iron and molybdenum homeostasis and impairs the cell envelope integrity. The results

dysfunction leads to a series of severe changes in E. coli cells.

Item Type: Article
DOI/Identification number: 10.1016/j.micres.2018.10.008
Uncontrolled keywords: E. coli, protein secretion, Twin-arginine translocation, proteomics, stress response
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Colin Robinson
Date Deposited: 19 Nov 2018 13:34 UTC
Last Modified: 23 Jan 2020 04:15 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/70187 (The current URI for this page, for reference purposes)
Robinson, Colin: https://orcid.org/0000-0002-1739-032X
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