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Cell-free synthetic biology for in vitro prototype engineering

Moore, Simon J., MacDonald, James T., Freemont, Paul S. (2017) Cell-free synthetic biology for in vitro prototype engineering. Biochemical Society Transactions, 45 (3). pp. 785-791. ISSN 0300-5127. E-ISSN 1470-8752. (doi:10.1042/BST20170011) (KAR id:69451)

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Abstract

Cell-free transcription-translation is an expanding field in synthetic biology as a rapid prototyping platform for blueprinting the design of synthetic biological devices. Exemplar efforts include translation of prototype designs into medical test-kits for onsite identification of viruses (Zika, Ebola), whilst gene circuit cascades can be tested, debugged and re-designed within rapid turnover times. Coupled with mathematical modelling, this discipline lends itself towards the precision engineering of new synthetic life. The next stages of cell-free look set to unlock new microbial hosts that remain slow to engineer and unsuited to rapid iterative design cycles. It is hoped that the development of such systems will provide new tools to aid the transition from cell-free prototype designs to functioning synthetic genetic circuits and engineered natural product pathways in living cells.

Item Type: Article
DOI/Identification number: 10.1042/BST20170011
Uncontrolled keywords: Cell-free, Prototyping, Synthetic Biology, Gene expression, Modelling
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Simon Moore
Date Deposited: 09 Oct 2018 09:18 UTC
Last Modified: 14 Jan 2020 10:23 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/69451 (The current URI for this page, for reference purposes)
Moore, Simon J.: https://orcid.org/0000-0002-1968-206X
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