Fiber bundle shifting endomicroscopy for high-resolution imaging

Vyas, Khushi and Hughes, Michael and Rosa, Bruno Gil and Yang, Guang-Zhong (2018) Fiber bundle shifting endomicroscopy for high-resolution imaging. Biomedical Optics Express, 9 (10). p. 4649. ISSN 2156-7085. (doi:https://doi.org/10.1364/BOE.9.004649) (Full text available)

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https://doi.org/10.1364/BOE.9.004649

Abstract

Flexible endomicroscopes commonly use coherent fiber bundles with high core densities to facilitate high-resolution in vivo imaging during endoscopic and minimally-invasive procedures. However, under-sampling due to the inter-core spacing limits the spatial resolution, making it difficult to resolve smaller cellular features. Here, we report a compact and rapid piezoelectric transducer (PZT) based bundle-shifting endomicroscopy system in which a super-resolution (SR) image is restored from multiple pixelation-limited images by computational means. A miniaturized PZT tube actuates the fiber bundle behind a GRIN micro-lens and a Delaunay triangulation based algorithm reconstructs an enhanced SR image. To enable real-time cellular-level imaging, imaging is performed using a line-scan confocal laser endomicroscope system with a raw frame rate of 120 fps, delivering up to 2 times spatial resolution improvement for a field of view of 350 µm at a net frame rate of 30 fps. The resolution enhancement is confirmed using resolution phantoms and ex vivo fluorescence endomicroscopy imaging of human breast specimens is demonstrated.

Item Type: Article
Uncontrolled keywords: endomicroscopy, imaging, resolution, fibre bundle
Subjects: Q Science > QC Physics > QC355 Optics
T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK8300 Optoelectronic devices
Divisions: Faculties > Sciences > School of Physical Sciences
Faculties > Sciences > School of Physical Sciences > Applied Optics Group
Depositing User: Michael Hughes
Date Deposited: 07 Sep 2018 09:56 UTC
Last Modified: 14 Sep 2018 09:55 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/68985 (The current URI for this page, for reference purposes)
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