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Fiber bundle shifting endomicroscopy for high-resolution imaging

Vyas, Khushi, Hughes, Michael, Rosa, Bruno Gil, Yang, Guang-Zhong (2018) Fiber bundle shifting endomicroscopy for high-resolution imaging. Biomedical Optics Express, 9 (10). Article Number 4664. ISSN 2156-7085. (doi:10.1364/BOE.9.004649) (KAR id:68985)

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Flexible endomicroscopes commonly use coherent fiber bundles with high core densities to facilitate high-resolution in vivo imaging during endoscopic and minimally-invasive procedures. However, under-sampling due to the inter-core spacing limits the spatial resolution, making it difficult to resolve smaller cellular features. Here, we report a compact and rapid piezoelectric transducer (PZT) based bundle-shifting endomicroscopy system in which a super-resolution (SR) image is restored from multiple pixelation-limited images by computational means. A miniaturized PZT tube actuates the fiber bundle behind a GRIN micro-lens and a Delaunay triangulation based algorithm reconstructs an enhanced SR image. To enable real-time cellular-level imaging, imaging is performed using a line-scan confocal laser endomicroscope system with a raw frame rate of 120 fps, delivering up to 2 times spatial resolution improvement for a field of view of 350 µm at a net frame rate of 30 fps. The resolution enhancement is confirmed using resolution phantoms and ex vivo fluorescence endomicroscopy imaging of human breast specimens is demonstrated.

Item Type: Article
DOI/Identification number: 10.1364/BOE.9.004649
Uncontrolled keywords: endomicroscopy, imaging, resolution, fibre bundle
Subjects: Q Science > QC Physics > QC355 Optics
T Technology > TK Electrical engineering. Electronics. Nuclear engineering > TK8300 Optoelectronic devices. Photoelectronic devices
Divisions: Divisions > Division of Natural Sciences > School of Physical Sciences
Depositing User: Michael Hughes
Date Deposited: 07 Sep 2018 09:56 UTC
Last Modified: 16 Feb 2021 13:57 UTC
Resource URI: (The current URI for this page, for reference purposes)
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