A novel live cell imaging system reveals a reversible hydrostatic pressure impact on cell cycle progression

Brooker, H. R. and Gyamfi, I. and Wieckowska, Agnieszka and Brooks, Nicholas J. and Mulvihill, Daniel P. and Geeves, Michael A. (2018) A novel live cell imaging system reveals a reversible hydrostatic pressure impact on cell cycle progression. Journal of Cell Science, . ISSN 0021-9533. (In press) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

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Abstract

Life is dependent upon the ability of a cell to rapidly respond to changes in environment. Small perturbations in local environments change the ability of molecules to interact and hence communicate. Hydrostatic pressure provides a rapid non-invasive, fully-reversible method for modulating affinities between molecules both in vivo and in vitro. We have developed a simple fluorescence imaging chamber that allows intracellular protein dynamics and molecular events to be followed at pressures up to 200 bar in living cells. Using yeast we investigate the impact of hydrostatic pressure upon cell growth and cell cycle progression. While 100 bar has no affect upon viability, it induces a delay in chromosome segregation, resulting in the accumulation of long-undividedbent cells, consistent with disruption of the cytoskeletons. This delay is independent of stress signalling and induces synchronisation of cell-cycle progression. Equivalent affects were observed in Candida albicans, with pressure inducing a reversible cell-cycle delay and hyphal growth. We present a simple novel non-invasive fluorescence microscopy based approach to transiently impact molecular dynamics to visualise, dissect and study signalling pathways and cellular processes in living cells.

Item Type:	Article
Uncontrolled keywords:	Fission yeast, live cell imaging, microscopy, cell synchonisation
Subjects:	Q Science Q Science > QR Microbiology
Divisions:	Faculties > Sciences > School of Biosciences
Depositing User:	Dan Mulvihill
Date Deposited:	07 Jun 2018 13:39 UTC
Last Modified:	08 Jun 2018 09:37 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/67231 (The current URI for this page, for reference purposes)
Brooker, H. R.:	https://orcid.org/0000-0001-5861-4759
Mulvihill, Daniel P.:	https://orcid.org/0000-0003-2502-5274
Geeves, Michael A.:	https://orcid.org/0000-0002-9364-8898