A novel live cell imaging system reveals a reversible hydrostatic pressure impact on cell cycle progression

Life is dependent upon the ability of a cell to rapidly respond to changes in

molecules to interact and hence communicate. Hydrostatic pressure provides

molecules both in vivo and in vitro. We have developed a simple fluorescence

events to be followed at pressures up to 200 bar in living cells. Using yeast we

progression. While 100 bar has no affect upon viability, it induces a delay in

cells, consistent with disruption of the cytoskeletons. This delay is

progression. Equivalent affects were observed in Candida albicans, with

a simple novel non-invasive fluorescence microscopy based approach to

transiently impact molecular dynamics to visualise, dissect and study signalling pathways and cellular processes in living cells.