Brooker, H. R., Gyamfi, I., Wieckowska, Agnieszka, Brooks, Nicholas J., Mulvihill, Daniel P., Geeves, Michael A. (2018) A novel live cell imaging system reveals a reversible hydrostatic pressure impact on cell cycle progression. Journal of Cell Science, 131 (15). Article Number 212167. ISSN 0021-9533. E-ISSN 1477-9137. (doi:10.1242/jcs.212167) (KAR id:67231)
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Official URL http://dx.doi.org/10.1242/jcs.212167 |
Abstract
Life is dependent upon the ability of a cell to rapidly respond to changes in
molecules to interact and hence communicate. Hydrostatic pressure provides
molecules both in vivo and in vitro. We have developed a simple fluorescence
events to be followed at pressures up to 200 bar in living cells. Using yeast we
progression. While 100 bar has no affect upon viability, it induces a delay in
cells, consistent with disruption of the cytoskeletons. This delay is
progression. Equivalent affects were observed in Candida albicans, with
a simple novel non-invasive fluorescence microscopy based approach to
transiently impact molecular dynamics to visualise, dissect and study signalling pathways and cellular processes in living cells.
Item Type: | Article |
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DOI/Identification number: | 10.1242/jcs.212167 |
Uncontrolled keywords: | Fission yeast, live cell imaging, microscopy, cell synchonisation |
Subjects: |
Q Science Q Science > QR Microbiology |
Divisions: | Divisions > Division of Natural Sciences > School of Biosciences |
Depositing User: | Daniel Mulvihill |
Date Deposited: | 07 Jun 2018 13:39 UTC |
Last Modified: | 05 Oct 2020 14:28 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/67231 (The current URI for this page, for reference purposes) |
Brooker, H. R.: | ![]() |
Mulvihill, Daniel P.: | ![]() |
Geeves, Michael A.: | ![]() |
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