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A novel live cell imaging system reveals a reversible hydrostatic pressure impact on cell cycle progression

Brooker, H. R., Gyamfi, I., Wieckowska, Agnieszka, Brooks, Nicholas J., Mulvihill, Daniel P., Geeves, Michael A. (2018) A novel live cell imaging system reveals a reversible hydrostatic pressure impact on cell cycle progression. Journal of Cell Science, 131 (15). Article Number 212167. ISSN 0021-9533. E-ISSN 1477-9137. (doi:10.1242/jcs.212167) (KAR id:67231)

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Abstract

Life is dependent upon the ability of a cell to rapidly respond to changes in

molecules to interact and hence communicate. Hydrostatic pressure provides

molecules both in vivo and in vitro. We have developed a simple fluorescence

events to be followed at pressures up to 200 bar in living cells. Using yeast we

progression. While 100 bar has no affect upon viability, it induces a delay in

cells, consistent with disruption of the cytoskeletons. This delay is

progression. Equivalent affects were observed in Candida albicans, with

a simple novel non-invasive fluorescence microscopy based approach to

transiently impact molecular dynamics to visualise, dissect and study signalling pathways and cellular processes in living cells.

Item Type: Article
DOI/Identification number: 10.1242/jcs.212167
Uncontrolled keywords: Fission yeast, live cell imaging, microscopy, cell synchonisation
Subjects: Q Science
Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Daniel Mulvihill
Date Deposited: 07 Jun 2018 13:39 UTC
Last Modified: 05 Oct 2020 14:28 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/67231 (The current URI for this page, for reference purposes)
Brooker, H. R.: https://orcid.org/0000-0001-5861-4759
Mulvihill, Daniel P.: https://orcid.org/0000-0003-2502-5274
Geeves, Michael A.: https://orcid.org/0000-0002-9364-8898
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