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The Long Non-Coding RNA Transcriptome Landscape in CHO Cells Under Batch and Fed-Batch Conditions

Vito, Davide, Smales, Christopher Mark (2018) The Long Non-Coding RNA Transcriptome Landscape in CHO Cells Under Batch and Fed-Batch Conditions. Biotechnology Journal, 13 (10). p. 1800122. ISSN 1860-6768. (doi:10.1002/biot.201800122) (KAR id:67183)

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https://doi.org/10.1002/biot.201800122

Abstract

The role of non?coding RNAs in determining growth, productivity and recombinant product quality attributes in Chinese hamster ovary (CHO) cells has received much attention in recent years, exemplified by studies into microRNAs in particular. However, other classes of non?coding RNAs have received less attention. One such class are the non?coding RNAs known collectively as long non?coding RNAs (lncRNAs). We have undertaken the first landscape analysis of the lncRNA transcriptome in CHO using a mouse based microarray that also provided for the surveillance of the coding transcriptome. We report on those lncRNAs present in a model host CHO cell line under batch and fed?batch conditions on two different days and relate the expression of different lncRNAs to each other. We demonstrate that the mouse microarray was suitable for the detection and analysis of thousands of CHO lncRNAs and validated a number of these by qRT?PCR. We then further analysed the data to identify those lncRNAs whose expression changed the most between growth and stationary phases of culture or between batch and fed?batch culture to identify potential lncRNA targets for further functional studies with regard to their role in controlling growth of CHO cells. We discuss the implications for the publication of this rich dataset and how this may be used by the community.

Item Type: Article
DOI/Identification number: 10.1002/biot.201800122
Uncontrolled keywords: Long non-coding RNAs (lncRNAs); Chinese hamster ovary (CHO) cells; microarray; transcriptome; recombinant protein production
Divisions: Faculties > Sciences > School of Biosciences > Protein Science Group
Depositing User: Mark Smales
Date Deposited: 31 May 2018 14:42 UTC
Last Modified: 09 Jul 2019 09:02 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/67183 (The current URI for this page, for reference purposes)
Smales, Christopher Mark: https://orcid.org/0000-0002-2762-4724
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