Skip to main content

Circular dichroism studies of low molecular weight hydrogelators: addressing practical issues

Sitsanidis, Efstratios D., Piras, Carmen C., Alexander, Bruce D., Siligardi, G., Javorfi, Tamas, Hall, Andrew J., Edwards, Alison A. (2018) Circular dichroism studies of low molecular weight hydrogelators: addressing practical issues. Chirality, . ISSN 0899-0042. (doi:10.1002/chir.22850)

PDF - Author's Accepted Manuscript
Download (1MB) Preview
[img]
Preview
Official URL
http://dx.doi.org/10.1002/chir.22850

Abstract

Circular dichroism (CD) spectroscopy has been used extensively for the investigation of the conformation and configuration of chiral molecules, but its use for evaluating the mode of self-assembly in soft materials has been limited. Herein, we report a protocol for the study of such materials by electronic CD spectroscopy using commercial/benchtop instruments and synchrotron radiation (SR) using the B23 beamline available at Diamond Light Source. The use of the B23 beamtime for SRCD was advantageous because of the unique enhanced spatial resolution achieved because of its highly collimated and small beamlight cross section (ca. 250 ?m) and higher photon flux in the far UV region (175-250 nm) enhancing the signal-to-noise ratio relative to benchtop CD instruments. A set of low molecular weight (LMW) hydrogelators, comprising two Fmoc-protected enantiomeric monosaccharides and one Fmoc dipeptide (Fmoc-FF), were studied. The research focused on the optimization of sample preparation and handling, which then enabled the characterization of sample conformational homogeneity and thermal stability. CD spectroscopy, in combination with other spectroscopic techniques and microscopy, will allow a better insight into the self-assembly of chiral building blocks into higher order structural architectures.

Item Type: Article
DOI/Identification number: 10.1002/chir.22850
Uncontrolled keywords: Circular dichroism, Self-assembly, Spectral profile, Supramolecular hydrogels
Subjects: Q Science
Q Science > QD Chemistry > QD431 Organic Chemistry- Biochemistry- Proteins, peptides, amino acids
Q Science > QD Chemistry > QD473 Physical properties in relation to structure
Q Science > QD Chemistry > Analytical Chemistry
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Andrew Hall
Date Deposited: 24 Apr 2018 15:01 UTC
Last Modified: 29 May 2019 20:29 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/66837 (The current URI for this page, for reference purposes)
Hall, Andrew J.: https://orcid.org/0000-0001-8849-7063
  • Depositors only (login required):

Downloads

Downloads per month over past year