Purification of lectin and Kunitz trypsin inhibitor from soya seeds

Roy, Uttam K. and Lavignac, Nathalie and Rahman, Aziz and Nielsen, Birthe V. (2018) Purification of lectin and Kunitz trypsin inhibitor from soya seeds. Journal of Chromatographic Science, 56 (5). pp. 436-442. ISSN 0021-9665. E-ISSN 1945-239X. (doi:https://doi.org/10.1093/chromsci/bmy018) (Full text available)

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https://doi.org/10.1093/chromsci/bmy018

Abstract

The search for potent and selective therapeutic agents is progressing by the study of natural compounds in plants. Plant-derived macromolecules are considered emerging therapeutic agents and an alternative to synthetic and small molecule drugs. Where it has long been known that plants possess medicinal properties, the compounds responsible for their action are in many cases still unknown: often only whole crude plant extracts or fractionated extracts are tested for the ability to inhibit common pathogens. Here, we present a fast protein liquid chromatography method for the separation of crude plant proteins. Kunitz trypsin inhibitor (KTI; 24.2 kDa) and lectin (31 kDa) were purified from Glycine max by liquid extraction followed by ion exchange column chromatography. The need for serial chromatographic separation steps has been eliminated by introducing more complex elution profiles hence reducing cost, time and improving recovery. The identity of KTI-A Q2 and lectin was confirmed by MALDI-TOF/TOF Mass Spectrometry. Cell proliferation assays using B16F1 melanoma cells revealed that both KTI and the monomeric lectin retained some antiproliferative activity. This method could be useful for rapid and cost-effective purification of bioactive compounds from plant material.

Item Type: Article
Uncontrolled keywords: Lectin, trypsin inhibitor, Glycine max, ion exchange chromatography
Subjects: Q Science > QD Chemistry > Analytical Chemistry
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Nathalie Lavignac
Date Deposited: 15 Mar 2018 13:39 UTC
Last Modified: 16 Mar 2019 00:00 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/66428 (The current URI for this page, for reference purposes)
Lavignac, Nathalie: https://orcid.org/0000-0002-9259-7603
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