Assessing embryo development using swept source optical coherence tomography

Caujolle, S. and Cernat, Ramona and Silvestri, Giuseppe and Marques, M.J. and Bradu, Adrian and Feuchter, Thomas and Robinson, Gary K. and Griffin, Darren K. and Podoleanu, Adrian G.H. (2018) Assessing embryo development using swept source optical coherence tomography. In: Podoleanu, Adrian G.H. and Bang, Ole, eds. Proceedings of SPIE. Proceedings of the 2nd Canterbury Conference on OCT with Emphasis on Broadband Optical Sources. SPIE p. 26. ISBN 978-1-5106-1674-5. (doi:https://doi.org/10.1117/12.2282912) (Full text available)

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Abstract

A detailed assessment of embryo development would assist biologists with selecting the most suitable embryos for transfer leading to higher pregnancy rates. Currently, only low resolution microscopy is employed to perform this assessment. Although this method delivers some information on the embryo surface morphology, no specific details are shown related to its inner structure. Using a Master-Slave Swept-Source Optical Coherence Tomography (SS-OCT), images of bovine embryos from day 7 after fertilization were collected from different depths. The dynamic changes inside the embryos were examined, in detail and in real-time from several depths. To prove our ability to characterize the morphology, a single embryo was imaged over 26 hours. The embryo was deprived of its life support environment, leading to its death. Over this period, clear morphological changes were observed.

Item Type: Conference or workshop item (Proceeding)
Uncontrolled keywords: Optical Coherence Tomography, Embryology, Biology, medical and biological imaging
Divisions: Faculties > Sciences > School of Physical Sciences
Faculties > Sciences > School of Physical Sciences > Applied Optics Group
Depositing User: S.M.C. Caujolle
Date Deposited: 13 Mar 2018 10:46 UTC
Last Modified: 13 Mar 2018 10:46 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/66359 (The current URI for this page, for reference purposes)
Marques, M.J.: https://orcid.org/0000-0002-7509-0704
Podoleanu, Adrian G.H.: https://orcid.org/0000-0002-4899-9656
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