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Assessing embryo development using swept source optical coherence tomography

Caujolle, S. and Cernat, R. and Silvestri, G. and Marques, M.J. and Bradu, A. and Feuchter, T. and Robinson, G. and Griffin, D. and Podoleanu, A. (2018) Assessing embryo development using swept source optical coherence tomography. In: Podoleanu, Adrian G.H. and Bang, Ole, eds. 2nd Canterbury Conference on OCT with Emphasis on Broadband Optical Sources. Proceedings of SPIE . SPIE. ISBN 978-1-5106-1674-5. E-ISBN 978-1-5106-1675-2. (doi:10.1117/12.2282912) (KAR id:66359)

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Abstract

A detailed assessment of embryo development would assist biologists with selecting the most suitable embryos for transfer leading to higher pregnancy rates. Currently, only low resolution microscopy is employed to perform this assessment. Although this method delivers some information on the embryo surface morphology, no specific details are shown related to its inner structure. Using a Master-Slave Swept-Source Optical Coherence Tomography (SS-OCT), images of bovine embryos from day 7 after fertilization were collected from different depths. The dynamic changes inside the embryos were examined, in detail and in real-time from several depths. To prove our ability to characterize the morphology, a single embryo was imaged over 26 hours. The embryo was deprived of its life support environment, leading to its death. Over this period, clear morphological changes were observed.

Item Type: Book section
DOI/Identification number: 10.1117/12.2282912
Uncontrolled keywords: Optical Coherence Tomography, Embryology, Biology, medical and biological imaging
Divisions: Faculties > Sciences > School of Physical Sciences > Applied Optics Group
Depositing User: S.M.C. Caujolle
Date Deposited: 13 Mar 2018 10:46 UTC
Last Modified: 06 Feb 2020 04:17 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/66359 (The current URI for this page, for reference purposes)
Marques, M.J.: https://orcid.org/0000-0002-7509-0704
Bradu, A.: https://orcid.org/0000-0002-6890-1599
Robinson, G.: https://orcid.org/0000-0003-2660-7778
Griffin, D.: https://orcid.org/0000-0001-7595-3226
Podoleanu, A.: https://orcid.org/0000-0002-4899-9656
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