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Development of lentiviral pseudotypes for surveillance studies on animal influenza viruses

Kinsley, Rebecca (2017) Development of lentiviral pseudotypes for surveillance studies on animal influenza viruses. Doctor of Philosophy (PhD) thesis, University of Kent,. (KAR id:66297)

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Abstract

Pseudotyped viruses (PVs) provide a safe, flexible platform for fundamental virological studies and antibody screening assays. Generation of influenza PVs involves co-transfection of producer cells with plasmids encoding the necessary viral components. The pseudotype virus neutralisation assay (PVNA) is a sensitive technique to measure protective antibody responses which cause neutralisation of virus particles. Many traditional methodologies, e.g. Haemagglutination Inhibition (HI) and Single Radial Haemolysis (SRH), detect only surface glycoprotein binding antibodies whereas the PVNA quantifies infectivity-neutralising responses.

Optimising PVs and employing them in a novel situation, such as an equine influenza vaccine efficacy trial, has been carried out with promising results. We have demonstrated that data obtained from the PVNA correlates well with the traditional SRH assay and consequently there is the potential for more widespread adoption in research and commercial settings. Furthermore, PVs have been manipulated to assess how single amino acid changes with equine influenza virus can affect the neutralisation efficacy of sera generated by vaccination.

Overall, PVs have been demonstrated as useful and readily-manipulated tools for studying antibody responses against equine, canine and phocine influenza viruses.

Item Type: Thesis (Doctor of Philosophy (PhD))
Thesis advisor: Scott, Simon
Uncontrolled keywords: pseudotyped viruses, animal influenza
Divisions: Faculties > Sciences > Medway School of Pharmacy
SWORD Depositor: System Moodle
Depositing User: System Moodle
Date Deposited: 06 Mar 2018 12:10 UTC
Last Modified: 01 May 2020 03:10 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/66297 (The current URI for this page, for reference purposes)
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