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Effect of bilayer charge on lipoprotein lipid exchange

Browning, Kathryn Louise, Lind, Tania Kjellerup, Maric, Selma, Barker, Robert, Cárdenas, Marité, Malmsten, Martin (2018) Effect of bilayer charge on lipoprotein lipid exchange. Colloids and Surfaces B: Biointerfaces, 168 . pp. 117-125. ISSN 0927-7765. (doi:10.1016/j.colsurfb.2018.01.043) (KAR id:66053)

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Lipoproteins play a key role in the onset and development of atherosclerosis, the formation of lipid plaques at blood vessel walls. The plaque formation, as well as subsequent calcification, involves not only endothelial cells but also connective tissue, and is closely related to a wide range of cardiovascular syndromes, that together constitute the number one cause of death in the Western World. High (HDL) and low (LDL) density lipoproteins are of particular interest in relation to atherosclerosis, due to their protective and harmful effects, respectively. In an effort to elucidate the molecular mechanisms underlying this, and to identify factors determining lipid deposition and exchange at lipid membranes, we here employ neutron reflection (NR) and quartz crystal microbalance with dissipation (QCM-D) to study the effect of membrane charge on lipoprotein deposition and lipid exchange. Dimyristoylphosphatidylcholine (DMPC) bilayers containing varying amounts of negatively charged dimyristoylphosphatidylserine (DMPS) were used to vary membrane charge. It was found that the amount of hydrogenous material deposited from either HDL or LDL to the bilayer depends only weakly on membrane charge density. In contrast, increasing membrane charge resulted in an increase in the amount of lipids removed from the supported lipid bilayer, an effect particularly pronounced for LDL. The latter effects are in line with previously reported observations on atherosclerotic plaque prone regions of long-term hyperlipidaemia and type 2 diabetic patients, and may also provide some molecular clues into the relation between oxidative stress and atherosclerosis.

Item Type: Article
DOI/Identification number: 10.1016/j.colsurfb.2018.01.043
Uncontrolled keywords: Atherosclerosis, HDL, LDL, Lipid exchange, Lipoprotein, Neutron Reflection
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > School of Physical Sciences
Depositing User: Robert Barker
Date Deposited: 19 Feb 2018 09:54 UTC
Last Modified: 16 Feb 2021 13:53 UTC
Resource URI: (The current URI for this page, for reference purposes)
Barker, Robert:
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