Skip to main content

Application of the SSB biosensor to study in vitro transcription

Cook, Alexander, Hari-Gupta, Yukti, Toseland, Christopher P. (2018) Application of the SSB biosensor to study in vitro transcription. Biochemical and Biophysical Research Communications, 496 (13). pp. 820-825. ISSN 0006-291X. (doi:10.1016/j.bbrc.2018.01.147) (KAR id:65865)

PDF Publisher pdf
Language: English

Click to download this file (1MB) Preview
[thumbnail of 1-s2.0-S0006291X18301682-main-1.pdf]
This file may not be suitable for users of assistive technology.
Request an accessible format
Official URL:


Gene expression, catalysed by RNA polymerases (RNAP), is one of the most fundamental processes in living cells. The majority of methods to quantify mRNA are based upon purification of the nucleic acid which leads to experimental inaccuracies and loss of product, or use of high cost dyes and sensitive spectrophotometers. Here, we describe the use of a fluorescent biosensor based upon the single stranded binding (SSB) protein. In this study, the SSB biosensor showed similar binding properties to mRNA, to that of its native substrate, single-stranded DNA (ssDNA). We found the biosensor to be reproducible with no associated loss of product through purification, or the requirement for expensive dyes. Therefore, we propose that the SSB biosensor is a useful tool for comparative measurement of mRNA yield following in vitro transcription.

Item Type: Article
DOI/Identification number: 10.1016/j.bbrc.2018.01.147
Uncontrolled keywords: Biosensor; Transcription; SSB; Myosin; RNA polymerase; Gene expression; mRNA
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Chris Toseland
Date Deposited: 02 Feb 2018 16:18 UTC
Last Modified: 09 Dec 2022 06:05 UTC
Resource URI: (The current URI for this page, for reference purposes)
  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.