Skip to main content

Rapid monitoring of recombinant protein products: a comparison of current technologies

Baker, Kym N., Rendall, Mark H., Patel, A., Boyd, Paul, Hoare, Michael, Freedman, Robert B., James, David C. (2002) Rapid monitoring of recombinant protein products: a comparison of current technologies. Trends Biotechnol, 20 (4). pp. 149-156. ISSN 0167-7799. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:6496)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=...

Abstract

Specific measurement of recombinant protein titer in a complex environment during industrial bioprocessing has traditionally relied on labor-intensive and time-consuming immunoassays. In recent years, however, developments in analytical technology have resulted in improved methods for protein product monitoring during bioprocessing. The choice of product-monitoring technology for a particular bioprocess will depend on a variety of assay factors and instrument-specific factors. In this article, we have compiled an overview of the advantages and disadvantages of the most commonly used technologies used: electrochemiluminescence, optical biosensors, rapid chromatography and nephelometry. The advantages of each technology for measuring both small and large recombinant therapeutic proteins are compared with a conventional enzyme-linked immunosorbent assay (ELISA) technique.

Item Type: Article
Additional information: 0167-7799 (Print) Comparative Study Journal Article
Uncontrolled keywords: Biosensing Techniques/economics/methods/trends Chemiluminescent Measurements Electrochemistry/economics/instrumentation/trends Enzyme-Linked Immunosorbent Assay/economics/methods/trends Humans *Molecular Probe Techniques Nephelometry and Turbidimetry/economics/methods/trends Optics/instrumentation Recombinant Proteins/*analysis
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences > Protein Science Group
Depositing User: Susan Davies
Date Deposited: 09 Sep 2008 18:10 UTC
Last Modified: 28 May 2019 13:42 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/6496 (The current URI for this page, for reference purposes)
  • Depositors only (login required):