Skip to main content

NDP52 activates nuclear myosin VI to enhance RNA polymerase II transcription

Fili, Natalia, Gupta, Yukti-Hari, dos Santos, Alia, Cook, Alexander, Poland, Simon, Ameer-Beg, Simon, Parsons, Maddy, Toseland, Christopher P. (2017) NDP52 activates nuclear myosin VI to enhance RNA polymerase II transcription. Nature Communications, 8 . 1871 (2017). ISSN 2041-1723. (doi:10.1038/s41467-017-02050-w) (KAR id:64916)

PDF Publisher pdf
Language: English

Creative Commons Licence
This work is licensed under a Creative Commons Attribution 4.0 International License.
Download (1MB) Preview
Official URL


Myosin VI (MVI) has been found to be overexpressed in ovarian, breast and prostate cancers. Moreover, it has been shown to play a role in regulating cell proliferation and migration, and to interact with RNA Polymerase II (RNAPII). Here, we find that backfolding of MVI regulates its ability to bind DNA and that a putative transcription co-activator NDP52 relieves the auto-inhibition of MVI to enable DNA binding. Additionally, we show that the MVI–NDP52 complex binds RNAPII, which is critical for transcription, and that depletion of NDP52 or MVI reduces steady-state mRNA levels. Lastly, we demonstrate that MVI directly interacts with nuclear receptors to drive expression of target genes, thereby suggesting a link to cell proliferation and migration. Overall, we suggest MVI may function as an auxiliary motor to drive transcription.

Item Type: Article
DOI/Identification number: 10.1038/s41467-017-02050-w
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Chris Toseland
Date Deposited: 30 Nov 2017 14:54 UTC
Last Modified: 29 May 2019 19:57 UTC
Resource URI: (The current URI for this page, for reference purposes)
  • Depositors only (login required):


Downloads per month over past year