A cell culture platform for Cryptosporidium that enables long-term cultivation and new tools for the systematic investigation of its biology

Miller, Christopher N. and Jossé, Lyne and Brown, Ian R. and Povey, Jane F. and Yiangou, Lyto and Price, Mark C. and Cinalt, Jindrich and Xue, Wei-Feng and Michaelis, Martin and Tsaousis, Anastasios D. (2018) A cell culture platform for Cryptosporidium that enables long-term cultivation and new tools for the systematic investigation of its biology. International Journal for Parasitology, 48 (3-4). pp. 197-201. ISSN 0020-7519. (doi:https://doi.org/10.1101/134270) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

PDF - Author's Accepted Manuscript

Creative Commons Licence
This work is licensed under a Creative Commons Attribution 4.0 International License.
Download (553kB) Preview
[img]
Preview
PDF (Accepted version of manuscript) - Author's Accepted Manuscript
Restricted to Repository staff only
Contact us about this Publication Download (352kB)
[img]
PDF - Publisher pdf
Download (993kB) Preview
[img]
Preview
Official URL
https://doi.org/10.1016/j.ijpara.2017.10.001

Abstract

Cryptosporidium parasites are a major cause of diarrhoea that pose a particular threat to children in developing areas and immunocompromised individuals. Curative therapies and vaccines are lacking, mainly due to lack of a long-term culturing system of this parasite. Here, we show that COLO-680N cells infected with two different Cryptosporidium parvum strains produce sufficient infectious oocysts to infect subsequent cultures, showing a substantial fold increase in production, depending on the experiment, over the most optimistic HCT-8 models. Oocyst identity was confirmed using a variety of microscopic- and molecular-based methods. This culturing system will accelerate research on Cryptosporidium and the development of anti-Cryptosporidium drugs.

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: Faculties > Sciences > School of Biosciences
Faculties > Sciences > School of Biosciences > Biomedical Research Group
Depositing User: Anastasios Tsaousis
Date Deposited: 20 Nov 2017 15:51 UTC
Last Modified: 12 May 2018 10:03 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/64370 (The current URI for this page, for reference purposes)
Tsaousis, Anastasios D.: https://orcid.org/0000-0002-5424-1905
  • Depositors only (login required):

Downloads

Downloads per month over past year