A cell culture platform for Cryptosporidium that enables long-term cultivation and new tools for the systematic investigation of its biology

Miller, Christopher N., Jossé, Lyne, Brown, Ian R., Povey, Jane F., Yiangou, Lyto, Price, Mark C., Cinalt, Jindrich, Xue, Wei-Feng, Michaelis, Martin, Tsaousis, Anastasios D. and others. (2018) A cell culture platform for Cryptosporidium that enables long-term cultivation and new tools for the systematic investigation of its biology. International Journal for Parasitology, 48 (3-4). pp. 197-201. ISSN 0020-7519. (doi:10.1101/134270) (KAR id:64370)

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Official URL: https://doi.org/10.1016/j.ijpara.2017.10.001

Abstract

Cryptosporidium parasites are a major cause of diarrhoea that pose a particular threat to children in developing areas and immunocompromised individuals. Curative therapies and vaccines are lacking, mainly due to lack of a long-term culturing system of this parasite. Here, we show that COLO-680N cells infected with two different Cryptosporidium parvum strains produce sufficient infectious oocysts to infect subsequent cultures, showing a substantial fold increase in production, depending on the experiment, over the most optimistic HCT-8 models. Oocyst identity was confirmed using a variety of microscopic- and molecular-based methods. This culturing system will accelerate research on Cryptosporidium and the development of anti-Cryptosporidium drugs.

Item Type:	Article
DOI/Identification number:	10.1101/134270
Subjects:	Q Science > QR Microbiology
Divisions:	Divisions > Division of Natural Sciences > Biosciences
Funders:	Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982)
Depositing User:	Lyne Josse
Date Deposited:	20 Nov 2017 15:51 UTC
Last Modified:	12 Jul 2022 10:41 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/64370 (The current URI for this page, for reference purposes)
Xue, Wei-Feng:	https://orcid.org/0000-0002-6504-0404
Michaelis, Martin:	https://orcid.org/0000-0002-5710-5888
Tsaousis, Anastasios D.:	https://orcid.org/0000-0002-5424-1905

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