Assay development and efficacy testing of novel and established antimicrobials

Over the past 25 years, new antimicrobial discovery has ground to a halt, this combined

effective treatments available to humankind decrease.

found them to be inefficient. One of the limiting factors for the discovery of new antimicrobial

by researchers. These methods mainly measure the ability of a compound to diffuse

Within this thesis I developed new testing assays for a family of novel antimicrobials, as

and MIC100 values of all the compounds in the group. They had measurable antimicrobial

Vancomycin, and measurable interaction with Gram-Negative bacteria, coating the cells

I then further explored the coating interaction, adding the compounds to bacteria, but also

efficacy of the established compounds and was also able to overcome resistance

Finally, I developed a modular, 3D printed robot to standardise and increase the accuracy

of the new testing methods.