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Does inflammation induced by ultraviolet B and heat rekindling alter pain-related behaviour in rats?

Smith, Melissa, Taylor, Collette, Weerasinghe, Nirosha, Koutsikou, Stella, Lumb, Bridget, Murrell, Jo (2016) Does inflammation induced by ultraviolet B and heat rekindling alter pain-related behaviour in rats? Veterinary Anaesthesia and Analgesia, 43 (5). pp. 579-585. ISSN 1467-2987. E-ISSN 1467-2995. (doi:10.1111/vaa.12349) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
https://doi.org/10.1111/vaa.12349

Abstract

OBJECTIVE: To investigate whether induction of the ultraviolet B and heat rekindling (UVB/HR) model alters burrowing behaviour in rats. STUDY DESIGN: Randomized, blinded, prospective experimental study. ANIMALS: Sixteen adult male Wistar rats weighing 250-300 g. METHODS: In the UVB/HR group (n = 8), UV irradiation was delivered to the heel area of the right plantar pelvic limb paw at a dose of 1000 mJ cm(-2) , using a narrow-band UVB light source. Twenty-four hours later, heat rekindling was performed by placement of a feedback-controlled thermode set at a constant temperature of 45 °C over the area of UVB irradiation for 5 minutes. Both interventions were carried out under pentobarbital anaesthesia. The 'sham' group (n = 8) was anaesthetized only. In the burrowing test, rats were housed singly for 2 hours in cages furnished with a burrow filled with sand. The amount of sand remaining in the burrow after 2 hours was weighed and the amount displaced from the burrow calculated. The burrowing test was carried out for two consecutive days prior to UVB irradiation (day 0), on day 1 prior to HR, on days 2 and 3 after UVB exposure and at equivalent time points in the sham group. RESULTS: Rats in the sham group burrowed means (SD) of 2429 (73) g and 2358 (124) g of sand on days -2 and 3, respectively, while those in the UVB/HR group burrowed 2460 (26) and 2419 (58) g on days -2 and 3, respectively. There was no significant effect of treatment on the amount of sand burrowed at any time point. CONCLUSIONS AND CLINICAL RELEVANCE: Pain associated with UVB/HR model induction is below the threshold required to affect rat burrowing behaviour and therefore questions the face validity of UVB/HR as a translational model of inflammatory pain.

Item Type: Article
DOI/Identification number: 10.1111/vaa.12349
Subjects: Q Science > QP Physiology (Living systems)
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Stella Koutsikou
Date Deposited: 24 Oct 2017 15:13 UTC
Last Modified: 29 May 2019 19:44 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/64136 (The current URI for this page, for reference purposes)
Koutsikou, Stella: https://orcid.org/0000-0003-2933-3637
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