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Speckle variance OCT for depth resolved assessment of the viability of bovine embryos

Caujolle, S., Cernat, Ramona, Silvestri, G., Marques, M.J., Bradu, Adrian, Feuchter, T., Robinson, Gary K., Griffin, Darren K., Podoleanu, Adrian G.H. (2017) Speckle variance OCT for depth resolved assessment of the viability of bovine embryos. Biomedical Optics Express, 8 (11). pp. 5139-5150. ISSN 2156-7085. (doi:10.1364/BOE.8.005139) (KAR id:64124)

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The morphology of embryos produced by in vitro fertilization (IVF) is commonly used to estimate their viability. However, imaging by standard microscopy is subjective and unable to assess the embryo on a cellular scale after compaction. Optical coherence tomography is an imaging technique that can produce a depth-resolved profile of a sample and can be coupled with speckle variance (SV) to detect motion on a micron scale. In this study, day 7 post-IVF bovine embryos were observed either short-term (10 minutes) or longterm (over 18 hours) and analyzed by swept source OCT and SV to resolve their depth profile and characterize micron-scale movements potentially associated with viability. The percentage of en face images showing movement at any given time was calculated as a method to detect the vital status of the embryo. This method could be used to measure the levels of damage sustained by an embryo, for example after cryopreservation, in a rapid and non-invasive way.

Item Type: Article
DOI/Identification number: 10.1364/BOE.8.005139
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Divisions > Division of Natural Sciences > School of Physical Sciences
Depositing User: Sophie Caujolle
Date Deposited: 23 Oct 2017 11:15 UTC
Last Modified: 16 Feb 2021 13:49 UTC
Resource URI: (The current URI for this page, for reference purposes)
Marques, M.J.:
Bradu, Adrian:
Robinson, Gary K.:
Griffin, Darren K.:
Podoleanu, Adrian G.H.:
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