The Use of Hyperimmune Chicken Reference Sera Is Not Appropriate for the Validation of Influenza Pseudotype Neutralization Assays

Ferrara, Francesca and Molesti, Eleonora and Scott, Simon D. and Cattoli, Giovanni and Temperton, Nigel J. (2017) The Use of Hyperimmune Chicken Reference Sera Is Not Appropriate for the Validation of Influenza Pseudotype Neutralization Assays. Pathogens, 6 (4). ISSN 2076-0817. E-ISSN 2076-0817. (doi:https://doi.org/10.3390/pathogens6040045) (Full text available)

Abstract

The pseudotype particle neutralization test (pp-NT) is a next-generation serological assay employed for the sensitive study of influenza antibody responses against hemagglutinin (HA), including stalk-directed antibodies. However, a validation of this assay has yet to be performed, and this limits its use to primarily research laboratories. To identify possible serological standards to be used in optimization and validation of the pp-NT, we have evaluated the cross-reactivity of hyperimmune chicken reference antisera in this assay. Our findings show that the cross-reactivity detected by the pp-NT is only partly explained by phylogenetic relationships and protein homology between the HA subtypes analysed; further studies are necessary to understand the origin of the cross-reactivity detected, and reference standards with higher specificity should be evaluated or generated de novo for future use in pp-NT.

Item Type:	Article
Uncontrolled keywords:	influenza; serology; standards; validation; pseudotypes; neutralization
Subjects:	Q Science > QR Microbiology > QR355 Virology
Divisions:	Faculties > Sciences > Medway School of Pharmacy
Depositing User:	Nigel Temperton
Date Deposited:	25 Sep 2017 15:34 UTC
Last Modified:	07 Dec 2017 10:31 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/63560 (The current URI for this page, for reference purposes)
Scott, Simon D.:	https://orcid.org/0000-0002-8290-0461
Temperton, Nigel J.:	https://orcid.org/0000-0002-7978-3815