Ferrara, Francesca, Molesti, Eleonora, Scott, Simon D., Cattoli, Giovanni, Temperton, Nigel J. (2017) The Use of Hyperimmune Chicken Reference Sera Is Not Appropriate for the Validation of Influenza Pseudotype Neutralization Assays. Pathogens, 6 (4). ISSN 2076-0817. E-ISSN 2076-0817. (doi:10.3390/pathogens6040045) (KAR id:63560)
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Official URL http://dx.doi.org/10.3390/pathogens6040045 |
Abstract
The pseudotype particle neutralization test (pp-NT) is a next-generation serological assay employed for the sensitive study of influenza antibody responses against hemagglutinin (HA), including stalk-directed antibodies. However, a validation of this assay has yet to be performed, and this limits its use to primarily research laboratories. To identify possible serological standards to be used in optimization and validation of the pp-NT, we have evaluated the cross-reactivity of hyperimmune chicken reference antisera in this assay. Our findings show that the cross-reactivity detected by the pp-NT is only partly explained by phylogenetic relationships and protein homology between the HA subtypes analysed; further studies are necessary to understand the origin of the cross-reactivity detected, and reference standards with higher specificity should be evaluated or generated de novo for future use in pp-NT.
Item Type: | Article |
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DOI/Identification number: | 10.3390/pathogens6040045 |
Uncontrolled keywords: | influenza; serology; standards; validation; pseudotypes; neutralization |
Subjects: | Q Science > QR Microbiology > QR355 Virology |
Divisions: | Faculties > Sciences > Medway School of Pharmacy |
Depositing User: | Nigel Temperton |
Date Deposited: | 25 Sep 2017 15:34 UTC |
Last Modified: | 29 May 2019 19:34 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/63560 (The current URI for this page, for reference purposes) |
Scott, Simon D.: | ![]() |
Temperton, Nigel J.: | ![]() |
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