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Assessment of the antigen-specific antibody response induced by mucosal administration of a GnRH conjugate entrapped in lipid nanoparticles

Gebril, Ayman M., Lamprou, Dimitrios A., Alsaadi, Manal M., Stimson, William H., Mullen, Alexander B., Ferro, Valerie A. (2013) Assessment of the antigen-specific antibody response induced by mucosal administration of a GnRH conjugate entrapped in lipid nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine, 10 (5). e971-e979. ISSN 1549-9634. (doi:10.1016/j.nano.2013.12.005) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

Abstract

Vaccines administered parenterally have been developed against gonadotrophin-releasing hormone (GnRH) for anti-fertility and anticancer purposes. The aim of this study was to demonstrate whether mucosal delivery using GnRH immunogens entrapped in lipid nanoparticles (LNP) could induce anti-GnRH antibody titers. Immunogens consisting of KLH (keyhole limpet hemocyanin) conjugated to either GnRH-I or GnRH-III analogues were entrapped in LNP. Loaded non-ionic surfactant vesicles (NISVs) were administered subcutaneously, while nasal delivery was achieved using NISV in xanthan gum and oral delivery using NISV containing deoxycholate (bilosomes). NISV and bilosomes had similar properties: they were spherical, in the nanometre size range, with a slightly negative zeta potential and surface properties that changed with protein loading and inclusion of xanthan gum. Following immunization in female BALB/c mice, systemic antibody responses were similar for both GnRH-I and GnRH-III immunization. Only nasal delivery proved to be successful in terms of producing systemic and mucosal antibodies.

Item Type: Article
DOI/Identification number: 10.1016/j.nano.2013.12.005
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Dimitrios Lamprou
Date Deposited: 19 Sep 2017 12:50 UTC
Last Modified: 29 May 2019 19:33 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/63488 (The current URI for this page, for reference purposes)
Lamprou, Dimitrios A.: https://orcid.org/0000-0002-8740-1661
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