Enzymatically activated emulsions stabilised by interfacial nanofibre networks

Moreira, Inês P., Sasselli, Ivan Ramos, Cannon, Daniel A., Hughes, Meghan, Lamprou, Dimitrios A., Tuttle, Tell, Ulijn, Rein V. (2016) Enzymatically activated emulsions stabilised by interfacial nanofibre networks. Soft Matter, 12 (9). pp. 2623-2631. ISSN 1744-683X. (doi:10.1039/c5sm02730f) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

PDF - Publisher pdf
Restricted to Repository staff only
Contact us about this Publication Download (1MB)
[img]
Official URL
https://doi.org/10.1039/c5sm02730f

Abstract

We report on-demand formation of emulsions stabilised by interfacial nanoscale networks. These are formed through biocatalytic dephosphorylation and self-assembly of Fmoc(9-fluorenylmethoxycarbonyl)-dipeptide amphiphiles in aqueous/organic mixtures. This is achieved by using alkaline phosphatase which transforms surfactant-like phosphorylated precursors into self-assembling aromatic peptide amphiphiles (Fmoc-tyrosine-leucine, Fmoc-YL) that form nanofibrous networks. In biphasic organic/aqueous systems, these networks form preferentially at the interface thus providing a means of emulsion stabilisation. We demonstrate on-demand emulsification by enzyme addition, even after storage of the biphasic mixture for several weeks. Experimental (Fluorescence, FTIR spectroscopy, fluorescence microscopy, electron microscopy, atomic force microscopy) and computational techniques (atomistic molecular dynamics) are used to characterise the interfacial self-assembly process.

Item Type: Article
DOI/Identification number: 10.1039/c5sm02730f
Subjects: R Medicine
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Dimitrios Lamprou
Date Deposited: 19 Sep 2017 12:38 UTC
Last Modified: 29 May 2019 19:33 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/63485 (The current URI for this page, for reference purposes)
Lamprou, Dimitrios A.: https://orcid.org/0000-0002-8740-1661
  • Depositors only (login required):

Downloads

Downloads per month over past year