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Nano-in-Nano Approach for Enzyme Immobilization Based on Block Copolymers

Auriemma, Finizia, De Rosa, Claudio, Malafronte, Anna, Di Girolamo, Rocco, Santillo, Chiara, Gerelli, Yuri, Fragneto, Giovanna, Barker, Robert, Pavone, Vincenzo, Maglio, Ornella, and others. (2017) Nano-in-Nano Approach for Enzyme Immobilization Based on Block Copolymers. ACS Applied Materials & Interfaces, 9 (34). pp. 29318-29327. ISSN 1944-8244. E-ISSN 1944-8252. (doi:10.1021/acsami.7b08959) (KAR id:62970)

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http://dx.doi.org/10.1021/acsami.7b08959

Abstract

We set up a facile approach for fabrication of supports with tailored nanoporosity for immobilization of enzymes. To this aim block copolymers (BCPs) self-assembly has been used to prepare nanostructured thin films with well-defined architecture containing pores of tailorable size delimited by walls with tailorable degree of hydrophilicity. In particular, we employed a mixture of polystyrene-block-poly(l-lactide) (PS-PLLA) and polystyrene-block-poly(ethylene oxide) (PS-PEO) diblock copolymers to generate thin films with a lamellar morphology consisting of PS lamellar domains alternating with mixed PEO/PLLA blocks lamellar domains. Selective basic hydrolysis of the PLLA blocks generates thin films, patterned with nanometric channels containing hydrophilic PEO chains pending from PS walls. The shape and size of the channels and the degree of hydrophilicity of the pores depend on the relative length of the blocks, the molecular mass of the BCPs, and the composition of the mixture. The strength of our approach is demonstrated in the case of physical adsorption of the hemoprotein peroxidase from horseradish (HRP) using 2,2?-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) with H2O2 as substrate. The large surface area, the tailored pore sizes, and the functionalization with hydrophilic PEO blocks make the designed nanostructured materials suitable supports for the nanoconfinement of HRP biomolecules endowed with high catalytic performance, no mass-transfer limitations, and long-term stability.

Item Type: Article
DOI/Identification number: 10.1021/acsami.7b08959
Uncontrolled keywords: block copolymers; enzyme immobilization; nanoporous surfaces; nanostructures; self-assembly
Subjects: Q Science
Divisions: Faculties > Sciences > School of Physical Sciences
Depositing User: Robert Barker
Date Deposited: 30 Aug 2017 09:37 UTC
Last Modified: 23 Jan 2020 04:13 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/62970 (The current URI for this page, for reference purposes)
Barker, Robert: https://orcid.org/0000-0002-8645-5385
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