High throughput automated microbial bioreactor system used for clone selection and rapid scale-down process optimization

Velez-Suberbie, M. Lourdes, Betts, John P. J., Walker, Kelly L., Robinson, Colin, Zoro, Barney, Keshavarz-Moore, Eli (2017) High throughput automated microbial bioreactor system used for clone selection and rapid scale-down process optimization. Biotechnology Progress, . ISSN 8756-7938. E-ISSN 1520-6033. (doi:10.1002/btpr.2534)

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Abstract

High throughput automated fermentation systems have become a useful tool in early bioprocess development. In this study, we investigated a 24 x 15 mL single use microbioreactor system, ambr 15f, designed for microbial culture. We compared the fed-batch growth and production capabilities of this system for two Escherichia coli strains, BL21 (DE3) and MC4100, and two industrially relevant molecules, hGH and scFv. In addition, different carbon sources were tested using bolus, linear or exponential feeding strategies, showing the capacity of the ambr 15f system to handle automated feeding. We used power per unit volume (P/V) as a scale criterion to compare the ambr 15f with 1 L stirred bioreactors which were previously scaled-up to 20 L with a different biological system, thus showing a potential 1,300 fold scale comparability in terms of both growth and product yield. By exposing the cells grown in the ambr 15f system to a level of shear expected in an industrial centrifuge, we determined that the cells are as robust as those from a bench scale bioreactor. These results provide evidence that the ambr 15f system is an efficient high throughput microbial system that can be used for strain and molecule selection as well as rapid scale-up

Item Type: Article
DOI/Identification number: 10.1002/btpr.2534
Uncontrolled keywords: E. coli; single use microbioreactor; high throughput; scale-down; ambr 15 fermentation
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH581.2 Cell Biology
Q Science > QR Microbiology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Colin Robinson
Date Deposited: 15 Aug 2017 11:20 UTC
Last Modified: 29 May 2019 19:21 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/62804 (The current URI for this page, for reference purposes)
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