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A case controlled study examining the bladder microbiome in women with Overactive Bladder (OAB) and healthy controls

Curtiss, Natasha, Balachandran, Aswini, Krska, Louise, Peppiatt-Wildman, Claire M., Wildman, Scott S.P., Duckett, Jonathan (2017) A case controlled study examining the bladder microbiome in women with Overactive Bladder (OAB) and healthy controls. European Journal of Obstetrics & Gynecology and Reproductive Biology, 214 . pp. 31-35. ISSN 0301-2115. (doi:10.1016/j.ejogrb.2017.04.040)

Abstract

Objective: To characterise the microbiome in healthy women with no bladder symptoms and to compare this to the bladder microbiome in patients with overactive bladder syndrome (OAB).

Study design: MSU specimens from 63 women with OAB were compared to urine from 35 controls. Urine was centrifuged and the resulting sediment pellet was re-suspended in supernatant and plated under aerobic conditions for 48 h and anaerobic conditions for 7 days. Each morphologically distinct colony was purity plated. Bacterial colonies were lysed and polymerase chain reaction undertaken to amplify the 16 s ribosomal RNA gene. This DNA was purified and sequenced allowing identification of bacterial genera.

Results: The mean number of different bacterial genera was 5.0 in both controls and OAB patients (p = 0.99). The uropathogenic bacteria Proteus (P = 0.01) was more commonly isolated from women with OAB. The genus lactobacillus was present less commonly in urine from OAB patients when compared to urine taken from controls (p = 0.02). Overall the most commonly grown bacteria were staphylococcus (grown in 59% of samples), streptococccus (51%), corynebacterium (37%) and lactobacillus (28%). A total of 95 different genera were identified from the urine samples.

Conclusion: The female human bladder has a diverse microbiome with stastistically significant differences between bacterial species present in OAB patients and controls.

Item Type: Article
DOI/Identification number: 10.1016/j.ejogrb.2017.04.040
Uncontrolled keywords: Microbiome, Overactive bladder, Women, Urinary incontinence
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Scott S.P. Wildman
Date Deposited: 07 Aug 2017 14:34 UTC
Last Modified: 29 May 2019 19:17 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/62576 (The current URI for this page, for reference purposes)
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