Protein Modifications during Antiviral Heat Bioprocessing and Subsequent Storage

Smales, C. Mark and Pepper, Duncan S. and James, David C. (2001) Protein Modifications during Antiviral Heat Bioprocessing and Subsequent Storage. Biotechnology Progress, 17 (5). pp. 974-978. ISSN 8756-7938 . (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Antiviral heat treatment is routinely used in the bioprocessing of therapeutic proteins as a means of reducing viral load. However, in protein formulations containing sucrose this form of bioprocessing can lead to protein modifications. Using a model protein, hen egg white lysozyme, we investigated the effects of antiviral heat treatments in the presence of sucrose on protein integrity during subsequent long-term protein storage. Although heat treatment alone resulted in protein modification, subsequent medium- to long-term storage of both lyophilized and liquid samples at room temperature or above led to further protein modifications. The majority of these modifications were due to the formation of glycation and advanced glycation end products via the reaction of reducing sugars and their autoxidation products (derived from hydrolyzed sucrose) with function groups on the protein surface. These findings have implications for the improvement of therapeutic protein bioprocessing to ensure protein product quality.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences
Depositing User: Mark Smales
Date Deposited: 28 Oct 2008 19:14
Last Modified: 23 May 2014 13:16
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