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Protein Modifications during Antiviral Heat Bioprocessing and Subsequent Storage

Smales, Christopher Mark, Pepper, Duncan S., James, David C. (2001) Protein Modifications during Antiviral Heat Bioprocessing and Subsequent Storage. Biotechnology Progress, 17 (5). pp. 974-978. ISSN 8756-7938. (doi:10.1021/bp010086f) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:6208)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
https://doi.org/10.1021/bp010086f

Abstract

Antiviral heat treatment is routinely used in the bioprocessing of therapeutic proteins as a means of reducing viral load. However, in protein formulations containing sucrose this form of bioprocessing can lead to protein modifications. Using a model protein, hen egg white lysozyme, we investigated the effects of antiviral heat treatments in the presence of sucrose on protein integrity during subsequent long-term protein storage. Although heat treatment alone resulted in protein modification, subsequent medium- to long-term storage of both lyophilized and liquid samples at room temperature or above led to further protein modifications. The majority of these modifications were due to the formation of glycation and advanced glycation end products via the reaction of reducing sugars and their autoxidation products (derived from hydrolyzed sucrose) with function groups on the protein surface. These findings have implications for the improvement of therapeutic protein bioprocessing to ensure protein product quality.

Item Type: Article
DOI/Identification number: 10.1021/bp010086f
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Mark Smales
Date Deposited: 28 Oct 2008 19:14 UTC
Last Modified: 09 Mar 2023 11:30 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/6208 (The current URI for this page, for reference purposes)

University of Kent Author Information

Smales, Christopher Mark.

Creator's ORCID: https://orcid.org/0000-0002-2762-4724
CReDIT Contributor Roles:

James, David C..

Creator's ORCID:
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