Candida glabrata Ste11 is involved in adaptation to hypertonic stress, maintenance of wild-type levels of filamentation and plays a role in virulence

Calcagno, Ana-Maria and Bignell, Elaine and Rogers, Thomas R. and Jones, Michael D. and Mühlschlegel, Fritz A. and Haynes, Ken (2005) Candida glabrata Ste11 is involved in adaptation to hypertonic stress, maintenance of wild-type levels of filamentation and plays a role in virulence. Medical Mycology, 43 (4). pp. 355-364. ISSN 1232-986X. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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The conserved family of fungal Ste11 mitogen activated protein kinase/kinases play important roles in several signalling cascades. We have cloned the STE11 homologue from the fungal pathogen Candida glabrata. The C. glabrata gene is present in a single copy in the genome, contains a well-conserved catalytic domain typical of the serine-threonine protein kinases and a sterile alpha motif widespread in signalling and nuclear proteins. Hypothetical translation of C. glabrata STE11 suggests that the protein has 64% identity and 77% similarity at the amino acid level to Saccharomyces cerevisiae Ste11. We have shown that C. glabrata STE11 can complement the mating defect and partially rescue the reduced nitrogen starvation induced filamentation of S. cerevisiae ste11 mutants. Functional analysis of a C. glabrata ste11 null mutant demonstrates that Ste11 is required for adaptation to hypertonic stress but is largely dispensable for maintenance of cell wall integrity. It also plays a role in C. glabrata nitrogen starvation induced filamentation. Survival analysis revealed that C. glabrata ste11 mutants, while still able to cause disease, are attenuated for virulence compared to reconstituted, STE11 cells. These data suggest that C. glabrata Ste11, in a similar fashion to the S. cerevisiae protein, functions in a number of different signalling modules.

Item Type: Article
Additional information: 1369-3786 (Print) Journal Article Research Support, Non-U.S. Gov't
Uncontrolled keywords: Amino Acid Sequence Animals Candida glabrata/genetics/growth & development/pathogenicity/*physiology Candidiasis/microbiology Fungal Proteins/chemistry/genetics/*metabolism Gene Expression Regulation, Fungal Heat-Shock Response MAP Kinase Kinase Kinases/chemistry/genetics/*metabolism Mice Molecular Sequence Data Morphogenesis Virulence
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Biomedical Research Group
Depositing User: F.A.M. Muhlschlegel
Date Deposited: 04 Sep 2008 22:21
Last Modified: 30 Apr 2014 10:50
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