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Candida glabrata Ste20 is involved in maintaining cell wall integrity and adaptation to hypertonic stress, and is required for wild-type levels of virulence

Calcagno, Ana-Maria, Bignell, Elaine, Rogers, Thomas R., Canedo, Mariana, Mühlschlegel, Fritz A., Haynes, Ken (2004) Candida glabrata Ste20 is involved in maintaining cell wall integrity and adaptation to hypertonic stress, and is required for wild-type levels of virulence. Yeast, 21 (7). pp. 557-568. ISSN 0749-503X. (doi:10.1002/yea.1125) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1002/yea.1125

Abstract

The conserved family of fungal Ste20 p21-activated serine-threonine protein kinases regulate several signalling cascades. In Saccharomyces cerevisiae Ste20 is involved in pheromone signalling, invasive growth, the hypertonic stress response, cell wall integrity and binds Cdc42, a Rho-like small GTP-binding protein required for polarized morphogenesis. We have cloned the STE20 homologue from the fungal pathogen Candida glabrata and have shown that it is present in a single copy in the genome. Translation of the nucleotide sequence predicts that C. glabrata Ste20 contains a highly conserved p21-activated serine-threonine protein kinase domain, a binding site for G-protein beta subunits and a regulatory Rho-binding domain that enables the kinase to interact with Cdc42 and/or Rho-like small GTPases. C. glabrata Ste20 has 53% identity and 58% predicted amino acid similarity to S. cerevisiae Ste20 and can complement both the nitrogen starvation-induced filamentation and mating defects of S. cerevisiae ste20 mutants. Analysis of ste20 null and disrupted strains suggest that in C. glabrata Ste20 is required for a fully functional hypertonic stress response and intact cell wall integrity pathway. C. glabrata Ste20 is not required for nitrogen starvation-induced filamentation. Survival analysis revealed that C. glabrata ste20 mutants, while still able to cause disease, are mildly attenuated for virulence compared to reconstituted STE20 cells.

Item Type: Article
DOI/Identification number: 10.1002/yea.1125
Additional information: 0749-503X (Print) Journal Article Research Support, Non-U.S. Gov't
Uncontrolled keywords: Amino Acid Sequence Animals Base Sequence Candida glabrata/enzymology/genetics/pathogenicity/*physiology Candidiasis/*microbiology Cell Wall/enzymology/physiology Cloning, Molecular DNA, Fungal/chemistry/genetics Fungal Proteins/genetics/*physiology Genetic Complementation Test Mice Molecular Sequence Data Mutagenesis, Insertional Polymerase Chain Reaction Protein-Serine-Threonine Kinases/genetics/*physiology Recombinant Proteins *Saccharomyces cerevisiae Proteins Sequence Alignment Virulence
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: F. Muhlschlegel
Date Deposited: 09 Sep 2008 16:04 UTC
Last Modified: 28 May 2019 13:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/6190 (The current URI for this page, for reference purposes)
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