Calcagno, Ana-Maria, Bignell, Elaine, Rogers, Thomas R., Canedo, Mariana, Mühlschlegel, Fritz A., Haynes, Ken (2004) Candida glabrata Ste20 is involved in maintaining cell wall integrity and adaptation to hypertonic stress, and is required for wild-type levels of virulence. Yeast, 21 (7). pp. 557-568. ISSN 0749-503X. (doi:10.1002/yea.1125) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:6190)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
Official URL: http://dx.doi.org/10.1002/yea.1125 |
Abstract
The conserved family of fungal Ste20 p21-activated serine-threonine protein kinases regulate several signalling cascades. In Saccharomyces cerevisiae Ste20 is involved in pheromone signalling, invasive growth, the hypertonic stress response, cell wall integrity and binds Cdc42, a Rho-like small GTP-binding protein required for polarized morphogenesis. We have cloned the STE20 homologue from the fungal pathogen Candida glabrata and have shown that it is present in a single copy in the genome. Translation of the nucleotide sequence predicts that C. glabrata Ste20 contains a highly conserved p21-activated serine-threonine protein kinase domain, a binding site for G-protein beta subunits and a regulatory Rho-binding domain that enables the kinase to interact with Cdc42 and/or Rho-like small GTPases. C. glabrata Ste20 has 53% identity and 58% predicted amino acid similarity to S. cerevisiae Ste20 and can complement both the nitrogen starvation-induced filamentation and mating defects of S. cerevisiae ste20 mutants. Analysis of ste20 null and disrupted strains suggest that in C. glabrata Ste20 is required for a fully functional hypertonic stress response and intact cell wall integrity pathway. C. glabrata Ste20 is not required for nitrogen starvation-induced filamentation. Survival analysis revealed that C. glabrata ste20 mutants, while still able to cause disease, are mildly attenuated for virulence compared to reconstituted STE20 cells.
Item Type: | Article |
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DOI/Identification number: | 10.1002/yea.1125 |
Additional information: | 0749-503X (Print) Journal Article Research Support, Non-U.S. Gov't |
Uncontrolled keywords: | Amino Acid Sequence Animals Base Sequence Candida glabrata/enzymology/genetics/pathogenicity/*physiology Candidiasis/*microbiology Cell Wall/enzymology/physiology Cloning, Molecular DNA, Fungal/chemistry/genetics Fungal Proteins/genetics/*physiology Genetic Complementation Test Mice Molecular Sequence Data Mutagenesis, Insertional Polymerase Chain Reaction Protein-Serine-Threonine Kinases/genetics/*physiology Recombinant Proteins *Saccharomyces cerevisiae Proteins Sequence Alignment Virulence |
Subjects: | Q Science |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | Fritz Muhlschlegel |
Date Deposited: | 09 Sep 2008 16:04 UTC |
Last Modified: | 05 Nov 2024 09:38 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/6190 (The current URI for this page, for reference purposes) |
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