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A novel higher plant protein tyrosine phosphatase interacts with SNF1-related protein kinases via a KIS (kinase interaction sequence) domain.

Fordham-Skelton, AP, Chilley, P, Lumbreras, V, Reignoux, S, Fenton, TR, Dahm, CC, Pages, M, Gatehouse, JA (2002) A novel higher plant protein tyrosine phosphatase interacts with SNF1-related protein kinases via a KIS (kinase interaction sequence) domain. The Plant journal : for cell and molecular biology, 29 (6). pp. 705-715. ISSN 0960-7412. (doi:10.1046/j.1365-313X.2002.01250.x) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1046/j.1365-313X.2002.01250.x

Abstract

A novel protein phosphatase in Arabidopsis thaliana was identified by database searching. This protein, designated AtPTPKIS1, contains a protein tyrosine phosphatase (PTP) catalytic domain and a kinase interaction sequence (KIS) domain. It is predicted to interact with plant SNF1-related kinases (SnRKs), representing central regulators of metabolic and stress responses. AtPTPKIS1 has close homologues in other plant species, both dicots and monocots, but is not found in other kingdoms. The tomato homologue of AtPTPKIS1 was expressed as a recombinant protein and shown to hydrolyse a generic phosphatase substrate, and phosphotyrosine residues in synthetic peptides. The KIS domain of AtPTPKIS1 was shown to interact with the plant SnRK AKIN11 both in vivo in the yeast two-hybrid system, and in vitro in a GST-fusion 'pull down' assay. The genomes of Arabidopsis and other plants contain further predicted proteins related to AtPTPKIS1, which could also interact with SnRKs and act in novel regulatory and signalling pathways.

Item Type: Article
DOI/Identification number: 10.1046/j.1365-313X.2002.01250.x
Uncontrolled keywords: Amino Acid Sequence, Arabidopsis, Arabidopsis Proteins, Conserved Sequence, Expressed Sequence Tags, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Intracellular Signaling Peptides and Proteins, Lycopersicon esculentum, Molecular Sequence Data, Protein Binding, Protein Tyrosine Phosphatases, Protein-Serine-Threonine Kinases, Sequence Homology, Amino Acid, Signal Transduction, Two-Hybrid System Techniques, Yeasts
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Tim Fenton
Date Deposited: 19 Dec 2018 06:44 UTC
Last Modified: 29 May 2019 19:00 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/61531 (The current URI for this page, for reference purposes)
Fenton, TR: https://orcid.org/0000-0002-4737-8233
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