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Protein kinase C phosphorylates ribosomal protein S6 kinase beta II and regulates its subcellular localization

Valovka, T, Verdier, F, Cramer, R, Zhyvoloup, A, Fenton, TR, Rebholz, H, Wang, ML, Gzhegotsky, M, Lutsyk, A, Matsuka, G, and others. (2003) Protein kinase C phosphorylates ribosomal protein S6 kinase beta II and regulates its subcellular localization. MOL CELL BIOL, 23 (3). 852 - 863. (doi:10.1128/MCB.23.3.852-863.2003) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:61528)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL
https://mcb.asm.org/content/23/3/852.short

Abstract

The ribosomal protein S6 kinase (S6K) belongs to the AGC family of Ser/Thr kinases and is known to be involved in the regulation of protein synthesis and the G(1),/S transition of the cell cycle. There are two forms of S6K, termed S6Kalpha and S6Kbeta, which have cytoplasmic and nuclear splice variants. Nucleocytoplasmic shuttling has been recently proposed for S6Kalpha, based on the use of the nuclear export inhibitor, leptomycin B. However, the molecular mechanisms regulating subcellular localization of S6Ks in response to mitogenic stimuli remain to be elucidated. Here we present data on the in vitro and in vivo phosphorylation of S6Kbeta, but not S6Kalpha, by protein kinase C (PKC). The site of phosphorylation was identified as S486, which is located within the C-terminal nuclear localization signal. Mutational analysis and the use of phosphospecific antibodies provided evidence that PKC-mediated phosphorylation at S486 does not affect S6K activity but eliminates the function of its nuclear localization signal and causes retention of an activated form of the kinase in the cytoplasm. Taken together, this study uncovers a novel mechanism for the regulation of nucleocytoplasmic shuttling of S6KbetaII by PKC-mediated phosphorylation.

Item Type: Article
DOI/Identification number: 10.1128/MCB.23.3.852-863.2003
Uncontrolled keywords: P70 S6 KINASE, MESSENGER-RNA TRANSLATION, NUCLEAR-LOCALIZATION, NUCLEOCYTOPLASMIC TRANSPORT, PHOSPHOINOSITIDE 3-KINASE, SIGNAL-TRANSDUCTION, PHORBOL ESTERS, CROSS-TALK, LATE PHASE, CELL-SIZE
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Tim Fenton
Date Deposited: 19 Dec 2018 06:30 UTC
Last Modified: 16 Nov 2021 10:24 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/61528 (The current URI for this page, for reference purposes)
Fenton, TR: https://orcid.org/0000-0002-4737-8233
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