CSN1 Somatic Mutations in Penile Squamous Cell Carcinoma

Feber, A and Worth, DC and Chakravarthy, A and de Winter, P and Shah, K and Arya, M and Saqib, M and Nigam, R and Malone, PR and Tan, WS and Rodney, S and Freeman, A and Jameson, C and Wilson, GA and Powles, T and Beck, S and Fenton, TR and Sharp, TV and Muneer, A and Kelly, JD (2016) CSN1 Somatic Mutations in Penile Squamous Cell Carcinoma. Cancer Research, 76 (16). pp. 4720-4727. ISSN 0008-5472. (doi:https://doi.org/10.1158/0008-5472.CAN-15-3134) (Full text available)

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http://dx.doi.org/10.1158/0008-5472.CAN-15-3134

Abstract

Other than an association with HPV infection, little is known about the genetic alterations determining the development of penile cancer. Although penile cancer is rare in the developed world, it presents a significant burden in developing countries. Here, we report the findings of whole-exome sequencing (WES) to determine the somatic mutational landscape of penile cancer. WES was performed on penile cancer and matched germline DNA from 27 patients undergoing surgical resection. Targeted resequencing of candidate genes was performed in an independent 70 patient cohort. Mutation data were also integrated with DNA methylation and copy-number information from the same patients. We identified an HPV-associated APOBEC mutation signature and an NpCpG signature in HPV-negative disease. We also identified recurrent mutations in the novel penile cancer tumor suppressor genes CSN1(GPS1) and FAT1. Expression of CSN1 mutants in cells resulted in colocalization with AGO2 in cytoplasmic P-bodies, ultimately leading to the loss of miRNA-mediated gene silencing, which may contribute to disease etiology. Our findings represent the first comprehensive analysis of somatic alterations in penile cancer, highlighting the complex landscape of alterations in this malignancy.

Item Type: Article
Additional information: Copyright © 2016 American Association for Cancer Research.
Uncontrolled keywords: science & technology, life sciences & biomedicine, oncology, human cancers, tumor types, protein, COP9, phosphorylation, deamination, expression, domain, P53
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Tim Fenton
Date Deposited: 21 Apr 2017 13:40 UTC
Last Modified: 20 Jun 2017 23:00 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/61502 (The current URI for this page, for reference purposes)
Fenton, TR: https://orcid.org/0000-0002-4737-8233
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