High speed, line-scanning, fiber bundle fluorescence confocal endomicroscopy for improved mosaicking

Hughes, Michael, Yang, Guang-Zhong (2015) High speed, line-scanning, fiber bundle fluorescence confocal endomicroscopy for improved mosaicking. Biomedical Optics Express, 6 (4). pp. 1241-1252. ISSN 2156-7085. (doi:10.1364/BOE.6.001241) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
https://doi.org/10.1364/BOE.6.001241

Abstract

A significant limitation of fiber bundle endomicroscopy systems is that the field of view tends to be small, usually only several hundred micrometers in diameter. Image mosaicking techniques can increase the effective image size, but require careful manipulation of the probe to ensure sufficient overlap between adjacent frames. For confocal endomicroscopes, which typically have frame rates on the order of 10 fps, this is particularly challenging. In this paper we demonstrate that line-scanning confocal endomicroscopy can, by use of a high speed linear CCD camera, achieve a frame rate of 120 fps while maintaining sufficient resolution and signal-to-noise ratio to allow imaging of topically stained gastrointestinal tissues. This leads to improved performance of a cross-correlation based mosaicking algorithm when compared with lower frame-rate systems.

Item Type: Article
DOI/Identification number: 10.1364/BOE.6.001241
Divisions: Faculties > Sciences > School of Physical Sciences
Faculties > Sciences > School of Physical Sciences > Applied Optics Group
Depositing User: Michael Hughes
Date Deposited: 06 Apr 2017 13:58 UTC
Last Modified: 29 May 2019 18:54 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/61185 (The current URI for this page, for reference purposes)
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