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Methylene-blue aided rapid confocal laser endomicroscopy of breast cancer

Vyas, Khushi, Hughes, Michael, Leff, Daniel Richard, Yang, Guang-Zhong (2017) Methylene-blue aided rapid confocal laser endomicroscopy of breast cancer. Journal of Biomedical Optics, 22 (2). 020501. ISSN 1083-3668. (doi:10.1117/1.JBO.22.2.020501) (KAR id:61179)

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https://doi.org/10.1117/1.JBO.22.2.020501

Abstract

Breast conserving surgery allows complete tumor resection while maintaining acceptable cosmesis for patients. Safe and rapid intraoperative margin assessment during the procedure is important to establish the completeness of tumor excision and minimizes the need for reoperation. Confocal laser endomicroscopy has demonstrated promise for real-time intraoperative margin assessment using acriflavine staining, but it is not approved for routine in-human use. We describe a custom high-speed line-scan confocal laser endomicroscopy (LS-CLE) system at 660 nm that enables high-resolution histomorphological imaging of breast tissue stained with methylene-blue, an alternative fluorescent stain for localizing sentinel nodes during breast surgery. Preliminary imaging results on freshly excised human breast tissue specimens are presented, demonstrating the potential of methylene-blue aided rapid LS-CLE to determine the oncological status of surgical margins in-vivo.

Item Type: Article
DOI/Identification number: 10.1117/1.JBO.22.2.020501
Divisions: Faculties > Sciences > School of Physical Sciences > Applied Optics Group
Depositing User: Michael Hughes
Date Deposited: 06 Apr 2017 14:06 UTC
Last Modified: 01 Aug 2019 10:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/61179 (The current URI for this page, for reference purposes)
Hughes, Michael: https://orcid.org/0000-0001-5301-2492
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