Skip to main content

Methylene-blue aided rapid confocal laser endomicroscopy of breast cancer

Vyas, Khushi, Hughes, Michael, Leff, Daniel Richard, Yang, Guang-Zhong (2017) Methylene-blue aided rapid confocal laser endomicroscopy of breast cancer. Journal of Biomedical Optics, 22 (2). Article Number 020501. ISSN 1083-3668. (doi:10.1117/1.JBO.22.2.020501) (KAR id:61179)

PDF Publisher pdf
Language: English

Download (520kB) Preview
[thumbnail of JBO_22_2_020501.pdf]
This file may not be suitable for users of assistive technology.
Request an accessible format
Official URL


Breast conserving surgery allows complete tumor resection while maintaining acceptable cosmesis for patients. Safe and rapid intraoperative margin assessment during the procedure is important to establish the completeness of tumor excision and minimizes the need for reoperation. Confocal laser endomicroscopy has demonstrated promise for real-time intraoperative margin assessment using acriflavine staining, but it is not approved for routine in-human use. We describe a custom high-speed line-scan confocal laser endomicroscopy (LS-CLE) system at 660 nm that enables high-resolution histomorphological imaging of breast tissue stained with methylene-blue, an alternative fluorescent stain for localizing sentinel nodes during breast surgery. Preliminary imaging results on freshly excised human breast tissue specimens are presented, demonstrating the potential of methylene-blue aided rapid LS-CLE to determine the oncological status of surgical margins in-vivo.

Item Type: Article
DOI/Identification number: 10.1117/1.JBO.22.2.020501
Divisions: Divisions > Division of Natural Sciences > School of Physical Sciences
Depositing User: Michael Hughes
Date Deposited: 06 Apr 2017 14:06 UTC
Last Modified: 16 Feb 2021 13:44 UTC
Resource URI: (The current URI for this page, for reference purposes)
Hughes, Michael:
  • Depositors only (login required):


Downloads per month over past year